The Role of Human Milk in Development of Breast Fed Child's Intestinal Microbiota (MYMILK)
The ideal food for normal infants is human milk. In addition, breast milk has been shown to be a continuous source of commensal, and⁄or probiotic bacteria to the infant gut where they play a key role in the initiation and development of the gut microbiota. Intestinal colonization is essential for maturation of the gut-associated lymphoid tissue (GALT) and the homeostasis of the intestinal epithelium. Colonization begins immediately after birth, while later the composition of the gut microbiota is affected by feeding practices. Results of some studies suggest that long-chain polyunsaturated fatty acids (LCPUFAs) promote the adhesion of probiotics to mucosal surfaces and along with probiotic bacteria contribute to the regulation of innate and adaptive immune responses and present a link among mother's diet, and microbes. The main purpose of the proposed research is to establish the link among mothers' nutrition, human milk LCPUFAs composition and microbiota and their potential influence on child's gut microbiota development. Pregnant women from three different regions of Slovenia will be involved into the study. Dietary intakes will be assessed during pregnancy, at the beginning of the 3rd trimester of pregnancy, and during lactation, at 4 weeks post partum, by 7-day weighed dietary protocol (7DP). Human milk (colostrum, and mature human milk) and infant's faeces will be sampled twice: at 2 or 3rd day post partum (meconium) and at 4 weeks post partum for the determination of human milk LCPUFAs composition and the assessment of human milk (colostrum und mature) and faeces microbiota. The fatty acid composition of human milk will be analysed by capillary gas-liquid chromatography (GC). Microbiota of milk and faeces will be studied using conventional microbiological and modern molecular approach such as qualitative and quantitative PCR amplification of informative genomic regions, DGGE/TGGE and/or t-RFLP and sequencing. Regular monthly meetings with the researchers will be organised for discussion and advising. Basic health parameters of infants will be followed. Data will be gathered from maternity department medical charts, from regular paediatric health care visits and from especially designed health care diary each mother will keep until the end of her child's first year.
|Study Design:||Observational Model: Cohort
Time Perspective: Prospective
|Official Title:||The Role of Human Milk in Development of Breast Fed Child's Intestinal Microbiota|
- Effect of nutrition patterns of pregnant and lactating women on fatty acid composition of milk and plasma lipids, vitemine D in blood, bone density and composition of microbiota of colostrum, milk, meconium and child's faeces [ Time Frame: from 27th-28th week of pregnancy to 12th-14th week post partum ] [ Designated as safety issue: Yes ]Nutrition patterns of pregnant and lactating women will be assessed by 4-day weighed dietary protocol (27.-28. week of pregnancy and 4 weeks after delivery). Fatty acids composition of milk, will be determined 4 weeks post partum and between 12th and 14th week post partum. Microbial composition of human colostrum, milk, child's meconium and faeces with emphasis on bifidobacteria and lactic acid bacteria will be determined 2nd- 3rd day post partum, at 4 weeks post partum and 12th-14th week post partum). The vitamin D and fatty acids in blood will be determined at 27th-37th week of pregnancy.
- Health and development of a child [ Time Frame: The first 12 months post partum ] [ Designated as safety issue: Yes ]Growth, type of nutrition, development and health status will be observed. The babies will be examined six times: at birth, at 1st month (between 28th and 38th day), at 3rd month (between 80th and 100th day), at 6th month (between 110th and 130th day), at 9th month (between 260th and 280th day) and at 12th month (between 355th and 375th day).The bone density will be determined at birth and at 1st, 3rd and 12th months.
Biospecimen Retention: Samples With DNA
colostrum, meconium, milk, child's faeces, urine, venous blood
|Study Start Date:||May 2010|
|Estimated Study Completion Date:||May 2013|
|Estimated Primary Completion Date:||May 2013 (Final data collection date for primary outcome measure)|
Pregnant women from Ljubljana region
Women at 3rd trimester of pregnancy living in the Ljubljana region.
Pregnant women from Izola region
Women at 3rd trimester of pregnancy living in the Izola region.
Pregnant women from Murska Sobota region
Women at 3rd trimester of pregnancy living in the Murska Sobota region.
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|University Medical Centre Ljubljana, Division of Paediatrics, Department of Neonatology|
|Ljubljana, Slovenia, 1000|
|Principal Investigator:||Irena Rogelj, Prof.||University of Ljubljana, Biotechnical faculty|