Serum Proteomics Analysis for Sepsis
Serum proteomics is a very useful tool to identify various disease. The purpose of the present study was to find differential proteins among patient with normal, SIRS, sepsis, severe sepsis, death and to screen potential biomarkers for their dynamic changes. Serum proteins were identified by iTRAQ labeling and LC-MS/MS. The bioinformatics analysis was performed with the Mascot software and the International Protein Index (IPI) and the Gene Ontology (GO) Database and KEGG pathway Database. The differentially expressed proteins were verified by Western blot by another sample collected from clinical.
|Study Design:||Time Perspective: Prospective|
|Official Title:||Serum Proteomics Analysis for Dynamic Changes of Differential Proteins in Sepsis Patient With iTRAQ Labeling and LC-MS/MS|
- Survival status [ Time Frame: 28 days after admission to ICU ] [ Designated as safety issue: No ]The survival time of patients more than 28days is defined as survival. The survival time of patients less than 28days is defined as death.
|Study Start Date:||May 2010|
|Estimated Study Completion Date:||March 2012|
|Estimated Primary Completion Date:||March 2012 (Final data collection date for primary outcome measure)|
normal person under physical examination
sepsis is defined as SIRS plus confirmed infection.
sepsis patients within 48 hours before death
|Contact: Longxiang Su, MD||+86 email@example.com|
|Chinese PLA General Hospital||Recruiting|
|Beijin, Beijing, China, 100853|
|Contact: Lixin Xie, MD firstname.lastname@example.org|
|Study Director:||Lixin Xie, MD||Department of Respiratory Diseases, Chinese PLA General Hospital|