Biomarkers in Blood and Tissue Samples From Patients With Epstein-Barr Virus-Positive Hodgkin Lymphoma
RATIONALE: Studying samples of blood and tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.
PURPOSE: This research trial studies biomarkers in blood and tissue samples from patients with Epstein-Barr virus positive Hodgkin lymphoma.
Genetic: DNA analysis
Genetic: RNA analysis
Genetic: cytogenetic analysis
Genetic: in situ hybridization
Genetic: mutation analysis
Genetic: polymerase chain reaction
Genetic: western blotting
Other: flow cytometry
Other: immunohistochemistry staining method
Other: medical chart review
|Official Title:||Role of the IL-2 Inducible Tcell Kinase in EBV-HLH and EBV+ Hodgkin's Lymphoma|
- Presence of germline ITK mutations [ Designated as safety issue: No ]
- Influence of ITK mutations on total expression levels or intracellular localization of the ITK protein [ Designated as safety issue: No ]
- Correlation between ITK mutations with specific clinical or histopathological features of HL [ Designated as safety issue: No ]
|Study Start Date:||December 2011|
|Estimated Primary Completion Date:||February 2012 (Final data collection date for primary outcome measure)|
- Evaluate germline DNA from patients with Epstein-Barr virus positive (EBV+) and Hodgkin lymphoma (HL) for inducible T-cell kinase (ITK) mutations.
- Examine the effects of ITK mutations on expression of the ITK protein.
- Determine whether ITK mutations correlate with specific clinical or histopathological features of HL.
OUTLINE: Archived blood and tumor tissue samples are analyzed for germline DNA expression and inducible T-cell kinase (ITK) mutations by PCR, IHC, flow cytometry, and western blotting, and EBV-encoded RNA (EBER) using in situ hybridization. Each patient's data, such as date, sex, age, tumor stage and histology at diagnosis, treatment received, response to treatment, development of recurrent disease, date of last follow-up, and outcomes are also collected.
|Principal Investigator:||Kim E. Nichols, MD, BA||Children's Hospital of Philadelphia|