The Effect of a Diabetic- and a Kidney-like Environment on Gene and Protein Expression in Cultured EC and SMC

This study is ongoing, but not recruiting participants.

Sponsor:

Information provided by (Responsible Party):

Meir Medical Center

ClinicalTrials.gov Identifier:

NCT01423279

First received: August 24, 2011

Last updated: February 7, 2013

Last verified: February 2013

History of Changes

Purpose

This research is NOT part of an interventional trial, however the investigators use umbilical cord after birth for isolating endothelial and smooth muscle cells for academic research.

Condition
Endothelial Dysfunction Disorder of Smooth Muscle

Study Type:	Observational
Official Title:	The Effect of a Diabetic- and a Kidney-like Environment on Gene and Protein Expression in Mono- and Co-cultured Endothelial and Smooth Muscle Cells

Further study details as provided by Meir Medical Center:

Primary Outcome Measures:

The effect of a Diabetic- and a Kidney-like environment on gene and protein expression in mono- and co-cultured endothelial and smooth muscle cells [ Time Frame: one year ] [ Designated as safety issue: No ]

Estimated Enrollment:	20
Study Start Date:	February 2013
Estimated Study Completion Date:	August 2014
Estimated Primary Completion Date:	January 2014 (Final data collection date for primary outcome measure)

Detailed Description:

Isolation and culturing of endothelial and smooth muscle muscles for academic research

Eligibility

Genders Eligible for Study:	Female
Accepts Healthy Volunteers:	Yes
Sampling Method:	Probability Sample

Study Population

Women after normal birth

Criteria

Inclusion Criteria:

normal pregnancy

Exclusion Criteria:

Contacts and Locations

Please refer to this study by its ClinicalTrials.gov identifier: NCT01423279

Locations

Israel
Laboratory of Renal Physiology, Meir Medical Center ,
Kfar Saba, Israel

Sponsors and Collaborators

Meir Medical Center

Investigators

Study Director:

Sydney Benchetrit, MD

Nephrology dept. Meir Medical Center, Kfar Saba , Israel

More Information

Publications:

Zitman-Gal T, Green J, Pasmanik-Chor M, Oron-Karni V, Bernheim J. Endothelial pro-atherosclerotic response to extracellular diabetic-like environment: possible role of thioredoxin-interacting protein. Nephrol Dial Transplant. 2010 Jul;25(7):2141-9. Epub 2010 Jan 19.

Talmor-Barkan Y, Bernheim J, Green J, Benchetrit S, Rashid G. Calcitriol counteracts endothelial cell pro-inflammatory processes in a chronic kidney disease-like environment. J Steroid Biochem Mol Biol. 2011 Mar;124(1-2):19-24. Epub 2011 Jan 12.

Talmor-Barkan Y, Rashid G, Weintal I, Green J, Bernheim J, Benchetrit S. Low extracellular Ca2+: a mediator of endothelial inflammation. Nephrol Dial Transplant. 2009 Nov;24(11):3306-12. Epub 2009 Jul 18.

Rashid G, Plotkin E, Klein O, Green J, Bernheim J, Benchetrit S. Parathyroid hormone decreases endothelial osteoprotegerin secretion: role of protein kinase A and C. Am J Physiol Renal Physiol. 2009 Jan;296(1):F60-6. Epub 2008 Oct 22.

Responsible Party:	Meir Medical Center
ClinicalTrials.gov Identifier:	NCT01423279 History of Changes
Other Study ID Numbers:	MMC11-0074CTIL, 0074-11-MMC
Study First Received:	August 24, 2011
Last Updated:	February 7, 2013
Health Authority:	Israel: Clalit Health Services

ClinicalTrials.gov processed this record on May 23, 2013

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