Study of Tumor Samples From Patients With Ependymoma Treated on the Children's Oncology Group ACNS0121 Trial
RATIONALE: Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. It may also help doctors find better ways to treat cancer.
PURPOSE: This research trial studies tumor samples from patients with ependymoma treated on the Children Oncology Group ACNS0121 trial.
Brain and Central Nervous System Tumors
Genetic: DNA analysis
Genetic: cytogenetic analysis
Genetic: fluorescence in situ hybridization
Genetic: gene expression analysis
Genetic: mutation analysis
Other: immunohistochemistry staining method
Other: laboratory biomarker analysis
|Official Title:||Examination of the Multiple Genetic and Molecular Targets as Therapeutic Options for Patients With Ependymoma Treated by the Phase II Children's Oncology Group Study ACNS0121|
- Association between OS and PFS with cell density, mitotic count, and tumor cell invasion [ Designated as safety issue: No ]
- Correlation between hTERT expression and telomere dysfunction and PFS and OS [ Designated as safety issue: No ]
- Role of 1q gain and 9p deletion and their association with PFS and OS [ Designated as safety issue: No ]
- Frequency and clinicopathological associations of mutations in genes involved in Notch pathway signaling [ Designated as safety issue: No ]
|Study Start Date:||March 2012|
|Estimated Primary Completion Date:||April 2013 (Final data collection date for primary outcome measure)|
- To examine the prognostic role of histopathological variables, in particular cellular density, mitotic count, and tumor cell invasion in intracranial pediatric ependymomas.
- To study whether hTERT expression and telomere dysfunction correlate with progression-free survival (PFS) and overall survival (OS) in pediatric intracranial ependymoma.
- To perform a genome-wide copy number screen and validation of copy number abnormalities (CNAs) on formalin-fixed paraffin-embedded (FFPE) ependymomas using Affymetrix Molecular Inversion Probe (MIP) arrays and interphase fluorescence in situ hybridization (iFISH).
- To evaluate associations between infiltration of immune markers and PFS as well as OS in pediatric ependymoma.
- To examine the role of 1q gain and 9p deletion in pediatric ependymomas by exploring their association with PFS and OS in a multivariable model.
- To establish the frequency and clinicopathological associations of mutations in genes involved in Notch pathway signaling.
OUTLINE: Archived tumor tissue samples are analyzed for cellular density, mitotic count, tumor cell invasion, hTERT expression, telomere dysfunction, 1q gain, 9p deletion, and genetic mutations by IHC, Affymetrix Molecular Inversion Probe (MIP) arrays, and fluorescence in situ hybridization (FISH). Results are then correlated with patient-outcome variables and known risk factors, namely gender, age at diagnosis, tumor location (infratentorial vs. supratentorial), tumor grade (differentiated vs anaplastic), and extent of surgery as well as pathologic variables.
|Principal Investigator:||Uri Tabori, MD||The Hospital for Sick Children|