DNA Double-strand Breaks After SPECT (DSB-SPECT)
Ionizing radiation has a number of harmful effects in humans. The most important among these is the induction of cancer. It is assumed that damage to DNA in the nucleus of a single cell can induce cancer. Among the different types of lesions inducted, DNA double-strand breaks (DSBs) are considered to be the most relevant effects that can initiate carcinogenesis.
The investigators are already conducting several other studies to prospectively compare the inducted DSBs by coronary CT-angiography and conventional coronary angiography. Extending these examinations to investigate the induced DSBs by myocardial scintigraphy allows a comparison of all three relevant imaging methods of the heart that incorporate ionizing radiation.
To evaluate this, the investigators are planning to examine patients who are scheduled for a clinically indicated myocardial scintigraphy. These examinations are routinely done by the Department of Nuclear Medicine in either a 1-day or a 2-day protocol according to the diagnostic reference values of the Federal Department for Radiological Protection. Blood samples will be taken from these patients at predefined time steps before and after the examination and DNA double-strand breaks will be determined from these blood samples specifically considering the applied activity of the tracer and the exposition kinetics.
|Study Design:||Observational Model: Cohort
Time Perspective: Prospective
|Official Title:||DNA Double-strand Breaks Following Myocardial Scintigraphy|
- Radiation-induced DNA double-strand breaks after myocardial scintigraphy. [ Time Frame: 48 hours ] [ Designated as safety issue: Yes ]DNA double-strand breaks will be measured before and up to 48 hours after radiation.
- Comparison of radiation-induced DSBs with activity used for myocardial scintigraphy. [ Time Frame: Activity will be measured 5 min and 1h after injection of the technetium tracer for SPECT. ] [ Designated as safety issue: No ]DNA double-strand breaks will be compared with injected activity.
|Study Start Date:||March 2011|
|Study Completion Date:||January 2013|
|Primary Completion Date:||January 2013 (Final data collection date for primary outcome measure)|
Patients clinically indicated to undergo myocardial SPECT in our institution.
Radiation: Myocardial SPECT
Myocardial SPECT according to clinical standards for patients with a clinical indication to undergo this imaging test.
Please refer to this study by its ClinicalTrials.gov identifier: NCT01380821
|Berlin, Germany, 10117|
|Principal Investigator:||Marc Dewey||Charité Berlin|