Essential Amino Acids Supplementation and Muscle Protein Synthesis

This study has been completed.
Sponsor:
Collaborator:
Tufts University
Information provided by:
United States Army Research Institute of Environmental Medicine
ClinicalTrials.gov Identifier:
NCT01366924
First received: June 1, 2011
Last updated: June 2, 2011
Last verified: June 2011
  Purpose

The modern warfighter faces numerous physiological challenges including sleep deprivation, sustained intense physical activity, and caloric restriction, the combined effects of which may result in the loss of lean body mass and decreased physical performance. Dietary interventions may help preserve lean body mass and facilitate recovery from periods of intense physical demand. For example, dietary strategies that increase amino acid availability have been shown to stimulate protein synthesis in skeletal muscle following resistance exercise. Because military tasks also incorporate endurance exercise components, studies regarding the effects of increasing dietary amino acids following endurance exercise are warranted. The objectives of this study are to characterize the effect of endurance exercise on protein synthesis and breakdown as well as the ability of an essential amino acid supplement to influence skeletal muscle protein metabolism and its cellular and molecular regulation following endurance exercise.


Condition Intervention Phase
Muscle Loss
Muscle Anabolism
Dietary Supplement: Essential Amino Acids
Other: Endurance exercise
Phase 1
Phase 2

Study Type: Interventional
Study Design: Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double Blind (Subject, Outcomes Assessor)
Primary Purpose: Basic Science
Official Title: The Effect of Amino Acid Supplementation on Skeletal Muscle Protein Turnover Following Endurance Exercise

Resource links provided by NLM:


Further study details as provided by United States Army Research Institute of Environmental Medicine:

Primary Outcome Measures:
  • Change in muscle protein synthesis after exercise or exercise with amino acid supplementation. [ Time Frame: An average change over a 210 minute recovery period from a 60 minute endurance exercise session ] [ Designated as safety issue: No ]

Secondary Outcome Measures:
  • Change in intracellular signaling after exercise or exercise with amino acid supplementation. [ Time Frame: At 30 minutes and 210 minutes after a 60 minute endurance exercise session ] [ Designated as safety issue: No ]

Enrollment: 23
Study Start Date: May 2007
Study Completion Date: July 2010
Primary Completion Date: July 2010 (Final data collection date for primary outcome measure)
Arms Assigned Interventions
No Intervention: Muscle protein turnover and intracellular signaling at rest
Muscle protein turnover and intracellular signaling are measured at rest for comparison to post-exercise muscle metabolism.
Active Comparator: Muscle metabolism after endurance exercise
Post-exercise muscle protein metabolism was measured to determine if endurance exercise affects muscle metabolism compared to rest.
Dietary Supplement: Essential Amino Acids
10 Gram Essential Amino Acid solutions with different leucine contents consumed during two identical endurance exercise trials
Other: Endurance exercise
60 minute endurance exercise session
Experimental: Muscle Metabolism after endurance exercise
Muscle metabolism response to endurance exercise with essential amino acid supplementation
Dietary Supplement: Essential Amino Acids
10 Gram Essential Amino Acid solutions with different leucine contents consumed during two identical endurance exercise trials
Experimental: Muscle anabolism after endurance exercise
Muscle anabolism after endurance exercise with essential amino acid supplementation.
Dietary Supplement: Essential Amino Acids
10 Gram Essential Amino Acid solutions with different leucine contents consumed during two identical endurance exercise trials

  Eligibility

Ages Eligible for Study:   18 Years to 35 Years
Genders Eligible for Study:   Both
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • Men and women aged 18 - 35 years
  • Weight stable
  • Recreationally active with VO2max of 40 - 50 ml/kg/min
  • Refrain from taking any NSAIDS or any aspirin-containing medications
  • Supervisor approval if a federal employee

Exclusion Criteria:

  • Disease or medication that affects macronutrient metabolism and/or the ability to participate in strenuous exercise
  • Allergies to foods or medications (including, but not limited to, lidocaine or phenylalanine) to be utilized in the study
  • Abnormal PT/PTT test or problems with blood clotting
  • Present condition of alcoholism or other substance abuse that compromises exercise capacity
  • Musculoskeletal injuries that compromise the ability to exercise
  • Pregnancy
  Contacts and Locations
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below. For general information, see Learn About Clinical Studies.

Please refer to this study by its ClinicalTrials.gov identifier: NCT01366924

Locations
United States, Massachusetts
Tufts University
Boston, Massachusetts, United States, 02111
US Army Research Institute of Environmental Medicine
Natick, Massachusetts, United States, 01760
Sponsors and Collaborators
United States Army Research Institute of Environmental Medicine
Tufts University
Investigators
Principal Investigator: Stefan M Pasiakos, Ph.D. US Army Research Institute of Environmental Medicine
  More Information

Publications:
Additional publications automatically indexed to this study by ClinicalTrials.gov Identifier (NCT Number):
Responsible Party: Stefan M Pasiakos/Research Physiologist, United States Army Research Institute of Environmental Medicine
ClinicalTrials.gov Identifier: NCT01366924     History of Changes
Other Study ID Numbers: H06-04, 7906 Tufts
Study First Received: June 1, 2011
Last Updated: June 2, 2011
Health Authority: United States: Federal Government

Keywords provided by United States Army Research Institute of Environmental Medicine:
protein turnover
whole body protein turnover
essential amino acids
leucine

ClinicalTrials.gov processed this record on August 28, 2014