Study of Methicillin-resistant Staphylococcus Aureus (MRSA) Isolated From Infected Patients in Brazil (MRSABrazil)

The recruitment status of this study is unknown because the information has not been verified recently.
Verified May 2011 by University of Sao Paulo.
Recruitment status was  Not yet recruiting
Sponsor:
Information provided by:
University of Sao Paulo
ClinicalTrials.gov Identifier:
NCT01359358
First received: May 20, 2011
Last updated: May 23, 2011
Last verified: May 2011
  Purpose

This project has as general objective to determine if there is any Methicillin-resistant Staphylococcus aureus (MRSA) strain disseminating in the hospital of Belo Horizonte. If this is the case, the investigators will determine if this is an international known strain by typing it by PFGE and MLST.

The detection of mecA gene will be performed by PCR. Their susceptibility profile to several drugs will also be accessed and it will be possible to compare the response to those drugs commonly in use to that of Daptomycin, a new drug available in Brazil.

It will be screened for reduced susceptibility to vancomycin by Macromethod Etest (MET) in order to search for VISA or hetero-VISA. Also, due to the discussions at the literature about mutations in genes that are said to be responsible for reduced susceptibility to vancomycin, the investigators will sequence these genes in all PFGE type strain of this study looking for mutations already described to compare to the screening results.


Condition
Methicillin Resistant Staphylococcus Aureus

Study Type: Observational
Study Design: Time Perspective: Retrospective
Official Title: Epidemiological Study of Methicillin-resistant Staphylococcus Aureus (MRSA) Isolated From Infected Patients During a Hospital Surveillance Period

Resource links provided by NLM:


Further study details as provided by University of Sao Paulo:

Estimated Enrollment: 20
Study Start Date: July 2011
Detailed Description:

Multidrug resistant and virulent MRSA strains have been disseminating worldwide and few alternatives of treatment for diseases caused by this pathogen are available. In this study, 36 isolates from colonization or different clinical specimens from infected patients of the Hospital Rizoleta Tolentino Neves will be analyzed. These results will provide important data for doctors and nurses, such as whether there is any strain dissemination or not in the hospital what could suggest an outbreak. It will be performed molecular typing by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The investigators will evaluate the efficiency of several antimicrobials and the frequency of h-VISA and VISA among the Brazilian MRSA. Besides, the investigators will verify the presence of mutation in genes that are involved in resistance mechanisms of VISA. According to the strains profile present in the hospital, if this is suggested that there is a dissemination of some strain, the health professionals can start a more rigid infection control measures in order to decrease the number of cases of nosocomial infection, decreasing the time of hospitalization of patients and, consequently the cost for the hospital.

  Eligibility

Ages Eligible for Study:   1 Month and older
Genders Eligible for Study:   Both
Accepts Healthy Volunteers:   No
Sampling Method:   Non-Probability Sample
Study Population

MRSA from infection or from colonization of a patient who was already infected by MRSA during the time of the study

Criteria

Inclusion Criteria:

  • MRSA from infection sites
  • MRSA from colonization of the patients who already was infected by MRSA during the time of the study

Exclusion Criteria:

  • MRSA from colonization of a patient who has not infection caused by MRSA
  Contacts and Locations
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below. For general information, see Learn About Clinical Studies.

Please refer to this study by its ClinicalTrials.gov identifier: NCT01359358

Contacts
Contact: Hyllo Baeta MArcelo Jr, Master degree 55 31 99929631 hyllo@lustosa.com.br

Locations
Brazil
Instituto de Física de Sao Carlos - Universidade de Sao Paulo Active, not recruiting
Sao Carlos, Sao Paulo, Brazil, 13560-590
Sponsors and Collaborators
University of Sao Paulo
Investigators
Principal Investigator: Ilana L B Camargo, PhD University of Sao Paulo
  More Information

No publications provided

Responsible Party: Ilana Lopes Baratella da Cunha Camargo, University of Sao Paulo
ClinicalTrials.gov Identifier: NCT01359358     History of Changes
Other Study ID Numbers: Novartis IFSC-USP
Study First Received: May 20, 2011
Last Updated: May 23, 2011
Health Authority: Brazil: Ethics Committee

Keywords provided by University of Sao Paulo:
MRSA
MLST
PFGE
Resistance
isolated from colonization

Additional relevant MeSH terms:
Staphylococcal Infections
Bacterial Infections
Gram-Positive Bacterial Infections

ClinicalTrials.gov processed this record on October 21, 2014