Branched-chain Amino Acids, and Resistance Exercise on Skeletal Muscle Signaling Pathways

This study has been completed.
Sponsor:
Information provided by (Responsible Party):
Humberto Nicastro, University of Sao Paulo
ClinicalTrials.gov Identifier:
NCT01350427
First received: May 2, 2011
Last updated: February 22, 2013
Last verified: February 2013
  Purpose

This study aims to evaluate the effects of leucine and branched-chain amino acids supplementation on skeletal muscle remodeling signaling pathways induced by resistance exercise.


Condition Intervention
Healthy
Dietary Supplement: Leucine supplementation
Dietary Supplement: BCAA supplementation
Dietary Supplement: Alanine supplementation

Study Type: Interventional
Study Design: Allocation: Randomized
Endpoint Classification: Safety/Efficacy Study
Intervention Model: Parallel Assignment
Masking: Double Blind (Subject, Investigator)
Primary Purpose: Basic Science
Official Title: Effects of Leucine, Branched-chain Amino Acids, and Resistance Exercise on Skeletal Muscle Remodelling Signaling Pathways: a Randomized, Double-blind, and Placebo-controlled Study

Resource links provided by NLM:


Further study details as provided by University of Sao Paulo:

Primary Outcome Measures:
  • Protein synthesis [ Time Frame: 1 hour ] [ Designated as safety issue: Yes ]
    Phosphorylation of proteins involved in protein synthesis

  • Protein degradation [ Time Frame: 1 hour ] [ Designated as safety issue: Yes ]
    Expression of protein involved in proteolytic pathway

  • Inflammation [ Time Frame: 1 hour ] [ Designated as safety issue: Yes ]
    Expression of systemic and muscular cytokines


Enrollment: 18
Study Start Date: August 2011
Study Completion Date: February 2013
Primary Completion Date: February 2013 (Final data collection date for primary outcome measure)
Arms Assigned Interventions
Experimental: Leucine Dietary Supplement: Leucine supplementation
1 bout of resistance exercise and supplementation of 4.0 g of leucine + 5.6 g of alanine
Experimental: BCAA Dietary Supplement: BCAA supplementation
1 bout of resistance exercise and supplementation of 4.0 g of leucine + 2.8 g of isoleucine + 2.8 g of valine
Placebo Comparator: Placebo Dietary Supplement: Alanine supplementation
1 bout of resistance exercise and supplementation of 9.6 g of alanine

  Eligibility

Ages Eligible for Study:   18 Years to 30 Years
Genders Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • Sedentaries
  • Must be able to exercise
  • Must be able to swallow tablets

Exclusion Criteria:

  • Use of nutritional supplements
  • Use of anti-inflammatory drugs
  Contacts and Locations
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below. For general information, see Learn About Clinical Studies.

Please refer to this study by its ClinicalTrials.gov identifier: NCT01350427

Locations
Brazil
University of São Paulo - School of Physical Education and Sports
São Paulo, Brazil, 05508-030
Sponsors and Collaborators
University of Sao Paulo
Investigators
Principal Investigator: Claudia R da Luz, Master Student University of São Paulo
  More Information

No publications provided

Responsible Party: Humberto Nicastro, PhD, University of Sao Paulo
ClinicalTrials.gov Identifier: NCT01350427     History of Changes
Other Study ID Numbers: 2011/04690-6
Study First Received: May 2, 2011
Last Updated: February 22, 2013
Health Authority: Brazil: Ethics Committee

Keywords provided by University of Sao Paulo:
leucine supplementation
BCCA supplementation
resistance exercise-induced muscle remodeling

ClinicalTrials.gov processed this record on July 31, 2014