Imatinib in KIT-negative Systemic Mastocytosis
The aim of this study is to evaluate the efficacy in terms of clinical and biological response rates of Imatinib Mesylate therapy in patients with systemic mastocytosis lacking KIT mutations.
|Study Design:||Endpoint Classification: Safety/Efficacy Study
Intervention Model: Single Group Assignment
Masking: Open Label
Primary Purpose: Treatment
|Official Title:||Imatinib Mesylate Therapy in Systemic Mastocytosis Patients Lacking KIT Mutations|
- To evaluate the effect of Imatinib Mesylate on clinical mastocytosis-related features: skin lesions, mast-cell related symptoms (pruritus, flushing, gastrointestinal symptoms, anaphylaxis), organomegalies/adenopathies and bone alterations. [ Time Frame: 12 months ] [ Designated as safety issue: No ]Clinical parameters are evaluated before and after therapy by macroscopic and histological skin examination, the record of frequency and severity of mast cell-mediators release symptoms, abdominal ultrasonography and x-ray survey.
- To evaluate the effect of Imatinib Mesylate on the grade of bone marrow mast cells infiltration as assessed by bone marrow histology and cytology, and flow cytometry performed on highly-purified bone marrow mast cells. [ Time Frame: 12 months ] [ Designated as safety issue: No ]
- To investigate changes after Imatinib Mesilate therapy in mast cell clonality as assessed by the HUMARA test performed on highly-purified bone marrow mast cells. [ Time Frame: 12 months ] [ Designated as safety issue: No ]
- To determine the effect of Imatinib Mesylate therapy on serum tryptase levels. [ Time Frame: 12 months ] [ Designated as safety issue: No ]
- To determine the effect of Imatinib Mesylate therapy in the psychological impact of the disease and the quality of life of patients as assessed by the Dermatology Life Quality Index . [ Time Frame: 12 months ] [ Designated as safety issue: No ]
|Study Start Date:||January 2011|
|Estimated Study Completion Date:||June 2012|
|Primary Completion Date:||December 2011 (Final data collection date for primary outcome measure)|
Drug: Imatinib Mesylate
- In patients without B or C findings and without biological progression: 300 mg/24 h p.o during one year or until progression/unacceptable toxicity.
- In patients with B or C findings or biological progression: 300 mg/24 h p.o for two weeks and then 400 mg/24 h p.o for a total of one year of therapy, or until progression/unacceptable toxicity.
In vitro studies have proven that imatinib inhibits wild type Kit (wtKit) and suppresses proliferation of the HMC-1V560G cell line, while it is ineffective on inhibiting the growth of HMC-1V560G, D816V cells. Apart from wtKit, Kit molecules carrying mutations in the extracellular, transmembrane and juxtamembrane domains, such as V560G, F522C and K509I, remain sensitive to imatinib. In contrast, several experiments have provided compelling evidence regarding the resistance against the growth-inhibitory effects of imatinib on cells carrying the D816V KIT mutation. As a consequence, sensitive and specific methods should be used in order to avoid "false" KIT mutation-negative cases and, for that purpose, mainly in cases with low bone marrow mast cell numbers, mutational studies should be performed using highly purified bone marrow mast cells by means of Facs sorting systems better than whole bone marrow, unsorted mononuclear cell fraction or mononuclear cell fraction pre-enriched using magnetic beads conjugated with anti-CD25 monoclonal antibody. In the present study mutational studies were performed in all cases in purified bone marrow mast cells (purity > 97%) using a FACSaria system (Becton-Dickinson Biosciences) as previously described.
Patients without B or C findings according to the World Health Organization, and without features of biological progression of the disease receive oral Imatinib Mesylate 300 mg daily for up to 12 months or until clinical progression/unacceptable toxicity. Patients with B or C findings or biological progression initially receive oral Imatinib Mesylate 300 mg daily for two weeks; then, dose is increased up to 400 mg/day except in patients who develop hematological or any other dose-limiting toxicity.
Biological progression is defined as the presence of at least one of the following features: i) increased serum tryptase levels > 200 ng/mL, ii) diffuse bone sclerosis, iii) patchy sclerosis with osteolysis and increased risk of bone fracture or significant bone pain, and, iv) organomegalies or lymph node enlargement due to mastocytosis.
|Instituto de Estudios de Mastocitosis de Castilla La Mancha; Hospital Virgen del Valle|
|Toledo, Spain, 45071|
|Principal Investigator:||Luis Escribano, MD, PhD||Instituto de Estudios de Mastocitosis de Castilla La Mancha|