Immune Responses After Human Subject Challenge With Sand Fly Bites
Recruitment status was Active, not recruiting
Sand flies can carry the infection leishmaniasis (a parasite). The purpose of this study is to evaluate the human immune response to uninfected laboratory raised sand fly bites and select from the immune response to sand fly saliva, possible substances to use for a future vaccine to protect against the parasite leishmaniasis.
|Study Design:||Observational Model: Cohort
Time Perspective: Prospective
|Official Title:||Immune Responses After Human Subject Challenge With Sand Fly Bites: Exploratory Study for Selection of Potential Leishmania Vaccine Antigen Candidates|
- Human Th1 immune response to specific sand fly salivary proteins [ Time Frame: 6-18 months ] [ Designated as safety issue: No ]Subject blood will be obtained at set points after repeated controlled sand fly feedings, duration for 20 minutes. Sand fly salivary molecules that demonstrate a strong Th1 immune response in these human PBMC, and little Th2 cytokine induction will be considered for development as future leishmania vaccine antigen candidates.
Biospecimen Retention: Samples With DNA
Plasma and white blood cells will be retained.
|Study Start Date:||October 2010|
|Estimated Study Completion Date:||December 2012|
|Estimated Primary Completion Date:||October 2012 (Final data collection date for primary outcome measure)|
Those in the Phlebotomus group will have exposure to P. duboscqui sand fly
Those placed in this group will receive exposure to L. longipalpis sand fly bites.
|United States, District of Columbia|
|Walter Reed Army Medical Center|
|Washington, District of Columbia, United States, 20307|