LANDA Embryo Freezing Technique.

The recruitment status of this study is unknown because the information has not been verified recently.
Verified September 2011 by West Coast Fertility Centers.
Recruitment status was  Active, not recruiting
Sponsor:
Information provided by (Responsible Party):
David G. Diaz, MD, West Coast Fertility Centers
ClinicalTrials.gov Identifier:
NCT01271400
First received: January 5, 2011
Last updated: September 6, 2011
Last verified: September 2011
  Purpose

The aim of this study is to track the outcome from the patients who had embryo cryopreservation as part of their Advanced Reproductive Technology (ART) treatment at West Coast Fertility Centers.


Condition
Primary Infertility
Secondary Infertility
Genital Diseases, Male
Genital Diseases, Female

Study Type: Observational
Study Design: Observational Model: Case-Only
Official Title: Use of the LANDA Embryo Freezing Technique to Validate Frozen Embryo Transfer Success Rates

Resource links provided by NLM:


Further study details as provided by West Coast Fertility Centers:

Primary Outcome Measures:
  • Freeze/Thawed survival rates [ Time Frame: When embryos thawed initiate ] [ Designated as safety issue: No ]
    • Freeze / thaw survival rates
    • Blastocyst formation rates
    • Pregnancy rate.


Estimated Enrollment: 20
Study Start Date: December 2010
Estimated Study Completion Date: January 2012
Estimated Primary Completion Date: January 2012 (Final data collection date for primary outcome measure)
Detailed Description:

Over the past several decades, considerable effort has been expended toward the successful cryopreservation of various human cells. While attempts at cryopreservation have been directed at different tissue types, one of the most vigorously pursued targets has been reproductive tissue. Historically, cryopreservation of human sperm has existed for several decades. The earliest reports of pregnancies (Trounson et al., 1983) and births (Zeilmaker et al., 1984) from the cryopreservation of human embryos occurred in the early 1980s. Presently, the freezing and storage of human embryos following in vitro fertilization (IVF) is standard practice at most fertility clinics. In 2003, the CDC Assisted Reproductive Technology success rates report stated that 4,246 live births occurred out of 17,517 non-donor frozen embryo cycles.

Embryo cryopreservation has been a routine component of clinical IVF programs for more than 2 decades but has a relatively poor outcome in terms of post-thaw survival and pregnancy rates in a majority of IVF programs. Efficient embryo cryopreservation has several advantages. It helps to reduce costs and increases cumulative pregnancy rates. It can also help in cases of IVF cycles where embryos are not transferred due to ovarian hyper stimulation syndrome. It is also useful when technical difficulties are encountered at the time of an embryo transfer procedure.

One of the major concerns in IVF is high-order multiple pregnancies, which result from the transfer of multiple embryos in a given cycle. In the last 5 years, improved stimulation protocols, advances in culture and laboratory systems, and better identification of viable embryos have enhanced the success rates of IVF. During the same period, some clinics have started the practice of transferring two embryos to reduce multiple pregnancy rates without compromising overall pregnancy rates.

Furthermore, in recent years some European countries, particularly the Scandinavian countries, have taken a lead in performing elective single-embryo transfers and have achieved acceptable pregnancy rates. This trend is spreading to other countries. This can result in surplus embryos being available for freezing. With the application of ICSI, even patients aged 40 years or older may have embryos to freeze.

Review of the current literature reveals modest post-thaw embryo survival, implantation, and pregnancy rates. Post-thaw survival rates vary from 50% to 80% for different embryo stages. Implantation and pregnancy rates have varied from 3% to 15% and 15% to 25% respectively, which is approximately half of the rates achieved for fresh embryo transfers.

To improve the outcome of frozen ET cycles, we modified various steps of our standard cryopreservation protocol. In a pilot study on arrested and fragmented embryos (grades 3 and 4), we achieved post-thaw survival rates of 92%, having all blastomeres intact. Encouraged by the post-thaw survival of these embryos; we propose using this modified protocol in our frozen embryo transfer program.

  Eligibility

Ages Eligible for Study:   21 Years to 37 Years
Genders Eligible for Study:   Female
Accepts Healthy Volunteers:   Yes
Sampling Method:   Non-Probability Sample
Study Population

Infertility patients

Criteria

Inclusion Criteria:

  • To be eligible for inclusion into this study, each subject must satisfy the following criteria:

    1. Infertile women whose physician has recommended IVF and who agree to cryopreserve surplus embryos.
    2. Cancer patients who wish to preserve their fertility prior to cancer treatments.
    3. Recipients who wish to use frozen donor embryos.
    4. A male partner whose semen analysis meets the clinic criteria for ICSI. Use of donor sperm is also acceptable.
    5. Be willing and able to comply with the protocol for the duration of the study.
    6. Have voluntarily provided written informed consent under WIRB.

Exclusion Criteria:

  • A patient may NOT be entered into the study if she presents with ANY of the following criteria:

    1. Clinically significant systemic disease.
    2. Known endometriosis Grade III - IV (ASRM classification).
    3. Any previous cycle indicating a low response to gonadotropin stimulation (defined as retrieval of < 10 eggs at retrieval)
    4. Three or more previous ART cycles without a clinical pregnancy
    5. Abnormal, undiagnosed, gynecological bleeding.
    6. Previous ovarian surgery
    7. Known allergy or hypersensitivity to recombinant gonadotropin preparations or any other study-related medications.
    8. Verifiable substance abuse.
    9. Simultaneous participation in another study protocol.
    10. Current smoker.
    11. An extrauterine pregnancy within the last 3 months before OCP treatment commences.
  Contacts and Locations
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below. For general information, see Learn About Clinical Studies.

Please refer to this study by its ClinicalTrials.gov identifier: NCT01271400

Locations
United States, California
West Coast Fertility Centers
Fountain Valley, California, United States, 92708
Sponsors and Collaborators
West Coast Fertility Centers
Investigators
Principal Investigator: David G. Diaz, M.D. West Coast Fertility Centers
  More Information

No publications provided

Responsible Party: David G. Diaz, MD, Medical Director, West Coast Fertility Centers
ClinicalTrials.gov Identifier: NCT01271400     History of Changes
Other Study ID Numbers: 2010-1
Study First Received: January 5, 2011
Last Updated: September 6, 2011
Health Authority: United States: Institutional Review Board

Keywords provided by West Coast Fertility Centers:
Infertility treatments
Frozen embryo transfers (FET).
IVF.
ART.
Cryopreservation
Slow-Freeze
Freeze / thaw survival rates
Blastocyst formation
Pregnancy

Additional relevant MeSH terms:
Infertility
Genital Diseases, Female
Genital Diseases, Male

ClinicalTrials.gov processed this record on September 18, 2014