Factors Involved in Obesity-related Inflammation and Insulin Resistance
In this project, we propose to recruit lean and obese subjects with different ethnic background (African Americans and Caucasians) to study the alterations of lipid and carbohydrate metabolism and determine whether these disturbances are linked to genetic, inflammatory, oxidative stress, and/or nutritional factors. Because systemic inflammation and insulin resistance are frequent features of obesity, we postulate that an unbalanced diet with high saturated- and low omega 3-fatty acids is linked to obesity-related inflammation and insulin resistance. We propose to investigate fatty acid metabolism and determine the links between fatty acid composition and oxidative stress in tissues of lean and obese subjects. We propose the following aims:
Specific Aim 1: Evaluate nutrient intake in lean and obese subjects using the standard NHANES Food Questionnaire.
Specific Aim 2: Evaluate the fatty acid composition, including omega-3, in adipose tissue depots, blood monocytes and skeletal muscle, and examine the relationship between omega-3 content and inflammatory and oxidative stress markers.
Specific Aim 3: Compare the effects of omega-3 and saturated FA supplementation on inflammatory and oxidative stress markers in vitro in adipose tissue explants, preadipocytes and monocyte culture.
|Study Design:||Observational Model: Case Control
Time Perspective: Cross-Sectional
|Official Title:||Genetic and Nutritional Factors Involved in Obesity-related Inflammation and Insulin Resistance|
- Examine the relationship between omega-3 content and inflammatory and oxidative stress markers [ Time Frame: 5 years ] [ Designated as safety issue: No ]We will evaluate fatty acid composition in adipose tissues depots, blood monocytes, and skeletal muscle to examine the relationship between omega-e content and inflammatory and oxidative stress markers.
- Evaluate nutrient intake [ Time Frame: 5 years ] [ Designated as safety issue: No ]Using the NHANES Food Questionnaire, we will evaluate nutrient intakes of lean and obese individuals
- Compare effects of omega-3 and saturated fatty acid supplementation on inflammatory and oxidative stress markers (in vitro) [ Time Frame: 5 years ] [ Designated as safety issue: No ]Compare the effects of omega-3 and saturated FA supplementation on inflammatory and oxidative stress markers in vitro in adipose tissue explants, preadipocytes and monocyte culture.
Biospecimen Retention: Samples With DNA
Whole blood, omentum biopsy, subcutaneous fat biopsy, muscle biopsy (abdominal wall)
|Study Start Date:||June 2011|
|Estimated Study Completion Date:||May 2016|
|Estimated Primary Completion Date:||May 2016 (Final data collection date for primary outcome measure)|
Normal weight: abdominal surgery
Lean individuals undergoing elective abdominal surgery
Obese: abdominal or bariatic surgery
Obese subjects undergoing elective abdominal or bariatric surgery
|United States, Tennessee|
|Vanderbilt University Medical Center|
|Nashville, Tennessee, United States, 37232|
|Principal Investigator:||Tahar Tamboli, PhD||Vanderbilt University|
|Study Chair:||Naji N Abumrad, MD||Vanderbilt University|