PD-1/PD-L1 Pathway Study on Septic Patients
The investigators want to identify that whether PD-1/PD-L1 pathway will change in human sepsis, and whether PD-1/PD-L1 pathway play an important role in sepsis induced immunosuppression.
|Study Design:||Observational Model: Case Control
Time Perspective: Cross-Sectional
|Official Title:||PD-1/PD-L1 Pathway in T Lymphocyte Apoptosis and Monocyte Dysfunction in Septic Patients|
peripheral venous blood from septic patients and healthy controls, anticoagulate with EDTA-K2
|Study Start Date:||December 2008|
|Study Completion Date:||April 2010|
|Primary Completion Date:||February 2010 (Final data collection date for primary outcome measure)|
First, we will detect PD-L1 expression on PBMC of septic patients and healthy controls.
Second, blockade of PD-1/PD-L1 pathway in vitro and stimulate with some biomolecules and then analyze the PBMC function and apoptosis by flow cytometry.
Then, compare with healthy controls and find the differences.