Mechanism of Aphakic and Pseudophakic Glaucoma in Pediatric Patients
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Purpose
In cell cultures, lens epithelial cells and trabecular meshwork cells will be exposed to aqueous humor from pediatric eyes undergoing cataract removal surgery. Cytokine analysis of candidate factors will be performed before and after exposure to lens epithelial.
| Condition |
|---|
|
Congenital Cataract Aphakic or Pseudophakic Glaucoma |
| Study Type: | Observational |
| Study Design: | Observational Model: Case-Only Time Perspective: Cross-Sectional |
| Official Title: | Mechanism of Aphakic and Pseudophakic Glaucoma in Pediatric Patients: Exploring the Pathophysiology Through Lens Epithelial Cell Culture and Isolation of Candidate Factors From Aqueous Humor |
| Estimated Enrollment: | 40 |
| Study Start Date: | July 2011 |
| Estimated Study Completion Date: | July 2013 |
| Estimated Primary Completion Date: | July 2013 (Final data collection date for primary outcome measure) |
| Groups/Cohorts |
|---|
| congenital cataract or aphakic glaucoma |
Detailed Description:
Aqueous from the anterior chamber will be withdrawn before surgery. Then the lens capsule will be opened (capsulorhexis) and removed. The lens material will be removed. All 3 components will be sent to the lab.
Lens epithelial cells will be isolated and will be used in a co-culture setup with trabecular cells to investigate the interactions between lens cells and trabecular cells. Understanding the interaction between young and adult lens cells on trabecular cell will shed light on mechanism of infantile aphakic glaucoma.
Aqueous humor taken out before cataract surgery and before glaucoma operation of aphakic eyes will be used for analysis of growth factor and cytokines in the Aphakic eye using proteins membrane arrays. Eyes before cataract surgery will be used as controls. Such analysis will shed light on the possible factors affecting trabecular cells in aphakic eye.
Eligibility| Ages Eligible for Study: | up to 18 Years |
| Genders Eligible for Study: | Both |
| Accepts Healthy Volunteers: | No |
| Sampling Method: | Non-Probability Sample |
children 0-18
Inclusion Criteria:
- children with congenital cataract and are going to do removal surgery
- children with aphakic or pseudophakic glaucoma and are going to do glaucoma surgery
- both of parents are able to sign the INFORMED CONSENT FORM
Exclusion Criteria:
- patients with other eye diseases apart from congenital cataract(in children with congenital cataract) and aphakic or pseudophakic glaucoma (in children with aphakic or pseudophakic glaucoma)
Contacts and Locations| Contact: Orna Geyer, Prof | 04-8250419 | orna_geyer@clalit.org.il |
| Israel | |
| Carmel Medical Center | Recruiting |
| Haifa, Israel | |
| Contact: Orna Geyer, Professor 97248250419 orna_geyer@clalit.org.il | |
| Principal Investigator: | Orna Geyer, Prof | Carmel Medical Center |
More Information
No publications provided
| Responsible Party: | Orna Geyer, Professor, Carmel Medical Center |
| ClinicalTrials.gov Identifier: | NCT01136473 History of Changes |
| Other Study ID Numbers: | CMC-10-0035-CTIL |
| Study First Received: | May 27, 2010 |
| Last Updated: | October 10, 2012 |
| Health Authority: | Israel: Ministry of Health |
Keywords provided by Carmel Medical Center:
|
congenital cataract aphakic pseudophakic glaucoma pediatric patients |
Additional relevant MeSH terms:
|
Cataract Glaucoma Lens Diseases Eye Diseases Ocular Hypertension |
ClinicalTrials.gov processed this record on June 17, 2013