Biomarkers in Tissue Samples From Patients With High-Risk Wilms Tumor
Recruitment status was Not yet recruiting
RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors to learn more about changes that occur in DNA and identify biomarkers related to cancer.
PURPOSE: This research study is studying biomarkers in tissue samples from patients with high-risk Wilms tumor.
Genetic: DNA methylation analysis
Genetic: gene expression analysis
Genetic: microarray analysis
Genetic: reverse transcriptase-polymerase chain reaction
Other: laboratory biomarker analysis
|Official Title:||Therapeutically Applicable Research to Generate Effective Treatments (TARGET) Initiative High-Risk Wilms Tumor Project: Application of Array-Based Methods and Next Generation Sequencing to Identify Candidate Molecular Targets for High-Risk Wilms Tumors|
- Genomic gains and losses in high-risk Wilms tumor [ Designated as safety issue: No ]
- Transcription patterns involved in the pathogenesis of Wilms tumor [ Designated as safety issue: No ]
- Genetic mutations involved in the pathogenesis of Wilms tumor [ Designated as safety issue: No ]
|Study Start Date:||March 2010|
|Estimated Primary Completion Date:||April 2010 (Final data collection date for primary outcome measure)|
- To assess genomic gains and losses in samples from high-risk Wilms tumor patients, including 50 favorable histology Wilms tumors that relapse (RFHWT) and 50 anaplastic Wilms tumors (UHWT) using a high-density genetic platform to survey for recurrent copy number variations and allelic imbalances.
- To define transcription patterns within these samples using a high-throughput platform for global gene expression.
- To define DNA methylation patterns within these samples using a high-throughput platform.
- To identify genetic mutations involved in the pathogenesis of Wilms tumor and in the development of relapse and anaplasia through the study of these samples using next-generation sequencing tools.
- To facilitate the integration of the above databases and allow meaningful access by investigators through the infrastructure provided by TARGET, including its data portal and associated caBIG tool.
OUTLINE: This is a multicenter study.
Archived tumor tissue samples are analyzed for DNA copy number determination, gene expression, DNA methylation, and genomic re-sequencing by array-based methods, including PCR analysis, methylation-specific reverse transcriptase-PCR (RT-PCR), and quantitative RT-PCR.
|Principal Investigator:||Elizabeth J. Perlman, MD||Ann & Robert H Lurie Children's Hospital of Chicago|