Chitinases and Transforming Growth Factor Beta (TGFB) in Human Asthma (AADCRC)
The purpose of this study is to find out the roles of two specific gene families (the chitinase gene family and the TGFB family). We hypothesize that chitinases and TGFb pathway genes will be differentially expressed in the airways of non-asthmatic subjects and subjects with asthma. We further hypothesize that genetic variants in CHIT1, AMCase, and TGFb pathway genes that show associations with asthma and related phenotypes will change the expression and/or function of the protein of these genes in the airway in several ways, including the transcript numbers for full length genes and splice variants and, for the chitinase genes, the levels of chitinase activity in airway secretions.
|Study Design:||Observational Model: Case Control
Time Perspective: Prospective
|Official Title:||Chitinases and TGFB in Human Asthma|
- Expression of CHIT1, AMCase, and TGFb pathway genes in asthma [ Time Frame: Current ] [ Designated as safety issue: No ]
Biospecimen Retention: Samples With DNA
sputum, blood, saliva, DNA, RNA, Plasma
|Study Start Date:||February 2010|
|Estimated Study Completion Date:||December 2013|
|Primary Completion Date:||December 2012 (Final data collection date for primary outcome measure)|
Healthy, non-asthmatic controls
People who are non-asthmatic and non-smokers.
People who have been diagnosed with asthma.
This is a cross-sectional study in patients with asthma and healthy controls in which we will analyze how the expression or function of CHIT1, AMCase, and TGFb pathway genes in the asthmatic airway is affected by genetic variation in these genes. We propose detailed phenotyping of the asthmatic subjects and the healthy controls, including collection of induced sputum, exhaled air and detailed physiologic measures including measures of airflow, lung volumes, and methacholine responsiveness as well as collection of airway cells and tissues by bronchoscopy. We will determine the relative expression of CHIT1 and AMCase in specific lung compartments (large airways, small airways, epithelial cells, macrophages) and the effects of genetic variants in CHIT1 and AMCase on expression of splice variants and levels of chitinase activity in the airway. These human samples will also allow us to determine if any of the multiple TGFb pathway genes analyzed show differential expression in the lung in asthma.
|Contact: Kelly Norsworthy, BAemail@example.com|
|United States, California|
|UCSF Airway Clinical Research Center||Recruiting|
|San Francisco, California, United States, 94143|
|Contact: Kelly Norsworthy, BA 415-502-8791 firstname.lastname@example.org|
|Contact: , BA|
|Principal Investigator: John V Fahy, MD, M.Sc.|
|Principal Investigator:||John V Fahy, MD||University of California, San Francisco|