Expansion of Umbilical Cord Blood Using a Unique Bio-system
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Purpose
By doing this study, researchers hope to develop a special technique and laboratory conditions to help support umbilical stem cell growth and multiplication.
In this project, researchers propose a three dimensional (3D) microenvironment that mimics the bone marrow stem cell microenvironment, with its supporting cells of osteoblasts and stromal cells. In theory, this will result in better expansion of cord blood stem cells ex vivo.
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Pregnancy |
| Study Type: | Observational |
| Official Title: | Ex Vivo Expansion of Umbilical Cord Blood Using a Unique Bio-system |
- bio-system for ex-vivo expansion of umbilical cord blood stem cells [ Time Frame: one year ] [ Designated as safety issue: No ]To successfully expand the umbilical cord blood stem cells, a microenvironment that resembles the stem cell microenvironment, or stem cell 'niche' should be created. In designing this bio-system, we make use of several observations. Firstly, bone osteoblasts are important in promoting hematopoietic stem cell expansion , accordingly, this bio-system will ensure the physical proximity of the cord blood stem cells to a 3D bone tissue derived from mesenchymal stem cells (MSCs). These MSCs will be isolated from Wharton's jelly, and by using an osteogenic medium and special scaffolds, these MSCs will differentiate into osteogenic progenitors creating a 3D bone structure . The other observation is that, co-culture of cord blood stem cells with mesenchymal stem cells is superior to liquid cultures in terms of ex vivo expansion , accordingly, in this experiment, undifferentiated MSCs will be co-cultured with cord blood stem cells in the created 3D bone structure.
Biospecimen Retention: Samples With DNA
umbilical cord
| Enrollment: | 8 |
| Study Start Date: | February 2010 |
| Study Completion Date: | November 2010 |
| Primary Completion Date: | November 2010 (Final data collection date for primary outcome measure) |
To successfully expand umbilical cord blood stem cells ex vivo, a microenvironment that resembles the stem cell microenvironment, or stem cell 'niche' should be created. In designing this bio-system, we make use of several observations. First, bone osteoblasts are important in promoting hematopoietic stem cell expansion, accordingly, this bio-system will ensure the physical proximity of the cord blood stem cells to a 3D bone tissue derived from mesenchymal stem cells (MSCs). These MSCs will be isolated from Wharton's jelly, and by using an osteogenic medium and special scaffolds, these MSCs will differentiate into osteogenic progenitors creating a 3D bone structure. The other observation is that, co-culture of cord blood stem cells with mesenchymal stem cells is superior to liquid cultures in terms of ex vivo expansion , accordingly, in this experiment, undifferentiated MSCs will be co-cultured with cord blood stem cells in this created 3D bone structure. This is a unique design that has the potential to expand cord blood stem cells more efficiently.
Eligibility| Ages Eligible for Study: | 18 Years and older |
| Genders Eligible for Study: | Female |
| Accepts Healthy Volunteers: | Yes |
| Sampling Method: | Probability Sample |
umbilical cord collection from full-term caesarian section/normal vaginal delivery patients
Inclusion Criteria:
- females that consent to permit researchers collect the discarded umbilical cords from the placenta and use for research purposes.
Exclusion Criteria:
-
Contacts and Locations| United States, Kansas | |
| University of Kansas Medical Center | |
| Kansas City, Kansas, United States, 66160 | |
| Principal Investigator: | Omar Aljitawi, MD | University of Kansas |
More Information
No publications provided
| Responsible Party: | University of Kansas |
| ClinicalTrials.gov Identifier: | NCT01061879 History of Changes |
| Other Study ID Numbers: | 11973 |
| Study First Received: | February 2, 2010 |
| Last Updated: | December 28, 2012 |
| Health Authority: | United States: Institutional Review Board |
Keywords provided by University of Kansas:
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umbilical cord umbilical cord blood collection pregnancy |
ClinicalTrials.gov processed this record on June 17, 2013