Tissue Sectioning by Electro-Dissociation

This study has been terminated.
(The study was closed due to the departure of several of the co-investigators.)
Information provided by:
University of Arkansas
ClinicalTrials.gov Identifier:
First received: December 21, 2007
Last updated: January 20, 2010
Last verified: January 2010

Currently there is no technique to produce thin (0.004-0.01 mm) serial sections of large fresh tissue specimens that are suitable for high-resolution in situ protein/gene expression studies without ice artifact or fixation-induced molecular damage. Traditional frozen sectioning preserves protein and nucleic acid structure, but the inherent ice artifact precludes reconstruction of protein and mRNA expression patterns in 3-dimensions. Since the limitations of the existing sectioning techniques result from the fact that they rely on mechanical cutting which in turn require the tissue to be stiff, we suggest a new approach to cut tissue via an electro erosion process that utilizes focus radio frequency (RF).

Condition Intervention
Thin Sections
Fresh Unfixed Tissues
Fresh Unfrozen Tissues
Other: Tissue Sectioning via Electro Erosion Process

Study Type: Observational
Official Title: Tissue Sectioning by Electro-Dissociation

Resource links provided by NLM:

Further study details as provided by University of Arkansas:

Enrollment: 0
Study Start Date: March 2002
Study Completion Date: January 2010
Primary Completion Date: January 2010 (Final data collection date for primary outcome measure)
Intervention Details:
    Other: Tissue Sectioning via Electro Erosion Process
    Focused RF energy can be used to produce consecutive thin sections of fresh tissue for immunohistochemical and nucleic acids analysis by electro-dissociation without thermal damage, ultimately allowing high resolution reconstruction of gene and protein expression patterns of large tissue specimens in 3D.

Genders Eligible for Study:   Both
Accepts Healthy Volunteers:   No
Sampling Method:   Probability Sample
Study Population

Discarded human tissue obtained immediately following surgical resection


Inclusion Criteria:

  • Fresh Tissue
  • Unfrozen Tissue
  • Unfixed Tissue

Exclusion Criteria:

  • Unfresh Tissue
  • Frozen Tissue
  • Fixed Tissue
  Contacts and Locations
Please refer to this study by its ClinicalTrials.gov identifier: NCT01054612

Sponsors and Collaborators
University of Arkansas
Principal Investigator: Gal Shafirstein, Ph.D. UAMS, ACH, Central Arkansas Veterans Healthcare System
  More Information

No publications provided

Responsible Party: Dr. Gal Shafirstein, University of Arkansas, Arkansas Children's Hospital
ClinicalTrials.gov Identifier: NCT01054612     History of Changes
Other Study ID Numbers: IRB 28177, NBIB
Study First Received: December 21, 2007
Last Updated: January 20, 2010
Health Authority: United States: Institutional Review Board

Keywords provided by University of Arkansas:
Gene Expression
Tissue Sectioning

Additional relevant MeSH terms:
Dissociative Disorders
Mental Disorders

ClinicalTrials.gov processed this record on April 17, 2014