Abnormal Expression Proteins, Mitochondrial DNA and miRNA of Irritable Bowel Syndrome (IBS)
Recruitment status was Active, not recruiting
In the investigators study, the investigators will focus on the screening of the related proteins and miRNA to IBS in order to reveal the possible clues or molecular mechanism for this disorder.
Irritable Bowel Syndrome
Digestive System Diseases
Colonic Diseases, Functional
|Study Design:||Observational Model: Case Control
Time Perspective: Prospective
|Official Title:||Screening and Identification of Abnormal Expression Proteins and miRNA From Colonic Mucosa of Patients With Irritable Bowel Syndrome.|
The investigators enrolled IBS-D and IBS-C patients on the Rome III criteria and healthy volunteers as normal control group. Biopsies were taken from the cecum and sigmoid colon under direct vision. Five pieces were taken separately at each site. Four pieces were processed by cold saline water and frozen in liquid nitrogen immediately while the other one was fixed in formalin and then embedded in paraffin three days later.
|Study Start Date:||January 2006|
|Estimated Study Completion Date:||December 2012|
|Estimated Primary Completion Date:||August 2011 (Final data collection date for primary outcome measure)|
For the proteomic procedures, Our study groups extracted proteins from the cecum and sigmoid colon samples and ran on the two-dimensional gel electrophoresis (2-DE). The proteins on 2-DE were visualized by the silver stain, and then gel maps were scanned and analyzed with the Image Master 2D Elite. The obvious abnormal protein spots were identified by the mass spectrometry. Western blot 、 immunohistochemistry 、 mRNA and miRNA were also performed for further verification of the proteins.
|Study Chair:||YANG Yun-sheng, MD||Chinese PLA General Hospital|
|Principal Investigator:||ZHANG Chun-yan, doctor||Chinese PLA General Hospital|
|Principal Investigator:||WANG Weifeng, doctor||Chinese PLA General Hospital|
|Principal Investigator:||YAO XIN, DOCTOR||Chinese PLA General Hospital|
|Principal Investigator:||PENG LIHUA, DOCTOR||Chinese PLA General Hospital|
|Principal Investigator:||YU YUANZI, DOCTOR||Chinese PLA General Hospital|