Study of Tissue and Blood Samples From Patients With Low-Grade Glioma
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Purpose
RATIONALE: Studying samples of tumor tissue and blood from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. It may also help doctors predict how patients will respond to treatment.
PURPOSE: This research study is looking at tissue and blood samples from patients with low-grade glioma.
| Condition | Intervention |
|---|---|
|
Brain and Central Nervous System Tumors |
Genetic: fluorescence in situ hybridization Genetic: loss of heterozygosity analysis Genetic: polymerase chain reaction Other: flow cytometry Other: immunohistochemistry staining method |
| Study Type: | Observational |
| Official Title: | Diagnostic and Prognostic Markers in Low-Grade Gliomas |
- Alterations of specific chromosomes and chromosomal regions including 7, 9p, 10p, 10q, 13q, 17p, 17q, 19q, 22q, X, and Y, using PCR analysis of microsatellite repeats and FISH [ Designated as safety issue: No ]
- DNA ploidy as determined by flow cytometric analysis; compare with ploidy determination by FISH [ Designated as safety issue: No ]
- Various markers of cellular proliferation and cellular function including flow cytometric determination of %S-phase, %G2M, and immunohistochemical evaluation of PCNA, Ki-67, and p53 [ Designated as safety issue: No ]
| Estimated Enrollment: | 237 |
OBJECTIVES:
- Evaluate the diagnostic and prognostic relevance of alterations of specific chromosomes and chromosomal regions including 7, 9p, 10p, 10q, 13q, 17p, 17q, 19q, 22q, X, and Y, using PCR analysis of microsatellite repeats and FISH.
- Evaluate the diagnostic and prognostic relevance of DNA ploidy by flow cytometric analysis; compare with ploidy determination by FISH.
- Assess the diagnostic and prognostic relevance of various markers of cellular proliferation and cellular function including flow cytometric determination of %S-phase, %G2M, and immunohistochemical evaluation of PCNA, Ki-67, and p53.
OUTLINE: Previously preserved paraffin-embedded tissue blocks are obtained and used for biomarker studies. Blood samples obtained during treatment are also obtained. Loss of heterozygosity of specific chromosomal regions are performed using PCR analysis of microsatellite repeats (41,118-120) on DNA extracted from the paraffin-embedded archival specimens. FISH and flow cytometry may also be used to assess chromosomal loss of deletion. Immunohistochemistry is also performed.
Eligibility| Genders Eligible for Study: | Both |
| Accepts Healthy Volunteers: | No |
DISEASE CHARACTERISTICS:
Diagnosis of low-grade glioma, meeting 1 of the following criteria:
- Paraffin embedded tumor tissue blocks obtained while enrolled in prospective NCCTG and Mayo studies available
- Paraffin-embedded tumor tissue blocks obtained while enrolled in NCCTG 86-72-51 or NCCTG 93-72-02 available
PATIENT CHARACTERISTICS:
- Not specified
PRIOR CONCURRENT THERAPY:
- See Disease Characteristics
Contacts and Locations
Show 38 Study Locations| Study Chair: | Jan C. Buckner, MD | Mayo Clinic |
| Investigator: | Robert M. Arusell, MD | Roger Maris Cancer Center at MeritCare Hospital |
| Investigator: | David W. Kimmel, MD | Mayo Clinic |
More Information
Additional Information:
No publications provided
| ClinicalTrials.gov Identifier: | NCT01004523 History of Changes |
| Other Study ID Numbers: | CDR0000406626, NCCTG-947253 |
| Study First Received: | October 29, 2009 |
| Last Updated: | January 18, 2013 |
| Health Authority: | Unspecified |
Keywords provided by National Cancer Institute (NCI):
|
childhood low-grade cerebellar astrocytoma childhood low-grade cerebral astrocytoma childhood mixed glioma adult mixed glioma |
adult diffuse astrocytoma childhood oligodendroglioma adult oligodendroglioma |
Additional relevant MeSH terms:
|
Glioma Nervous System Neoplasms Central Nervous System Neoplasms Neoplasms, Neuroepithelial Neuroectodermal Tumors Neoplasms, Germ Cell and Embryonal |
Neoplasms by Histologic Type Neoplasms Neoplasms, Glandular and Epithelial Neoplasms, Nerve Tissue Neoplasms by Site Nervous System Diseases |
ClinicalTrials.gov processed this record on June 18, 2013