Embryo culture is a cornerstone of in vitro fertilization treatments. Usually, embryos are cultured in microdrops of culture media in incubators with controlled temperature, humidity and atmosphere. However, these culture systems are static, while in vivo the initial growth of the embryo takes place in the uterine tube and they are submitted to a dynamic environment. The aim of this study is to compare the embryo development on days 2 and 3 of culture morphological features of embryos under static and dynamic(microfluidics) systems of culture.
Primary Outcome Measures:
- Embryo morphology on days 2 and 3 of embryo culture according to the criteria described by Lucinda Veeck [ Time Frame: Embryo culture for 3 days before transference to the uterus ] [ Designated as safety issue: No ]
Secondary Outcome Measures:
- Implantation rate and pregnancy rate [ Time Frame: 24 month ] [ Designated as safety issue: No ]
| Estimated Enrollment: |
400 |
| Study Start Date: |
October 2009 |
| Estimated Study Completion Date: |
October 2011 |
| Estimated Primary Completion Date: |
October 2010 (Final data collection date for primary outcome measure) |
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Active Comparator: Static culture
Embryos individually cultured in microdrops of 40 microliters of G-IVFplus series V
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Device: Static culture
Embryos cultured in 40 microliters microdrops of G-IVF plus series V
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Experimental: Smart plataform
Embryos culture in dynamics microfluidic culture system
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Device: SMART dynamic system of embryo culture
Embryos will be cultured in a dynamic system of microfluidics.
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Embryo culture is a cornerstone of in vitro fertilization treatments. Usually, embryos are cultured in microdrops of culture media in incubators with controlled temperature, humidity and atmosphere. However, these culture systems are static, while in vivo the initial growth of the embryo takes place in the uterine tube and they are submitted to a dynamic environment. The aim of this study is to compare the embryo development on days 2 and 3 of culture morphological features of embryos under static and dynamic(microfluidics) systems of culture.
Purpose
