DNA Analysis of Tissue From Patients With T-Cell Acute Lymphoblastic Leukemia
Recruitment status was Not yet recruiting
RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.
PURPOSE: This laboratory study is looking at tissue samples from patients with T-cell acute lymphoblastic leukemia.
Genetic: microarray analysis
Genetic: mutation analysis
Other: immunologic technique
|Official Title:||Molecular Mechanisms of NOTCH Induced Transformation in T-ALL|
- Transcriptional signatures associated with NOTCH1 mutations in T-cell acute lymphoblastic leukemia (T-ALL) cells [ Designated as safety issue: No ]
- Global changes on gene expression resulting from inactivation of NOTCH signaling in T-ALL cells [ Designated as safety issue: No ]
|Study Start Date:||December 2006|
- Identify the transcriptional signatures associated with the presence of NOTCH1 mutations in primary T-cell acute lymphoblastic leukemia (T-ALL) cells using oligonucleotide microarrays.
- Characterize the global changes on gene expression resulting from the inactivation of NOTCH signaling in human T-ALL lymphoblasts.
OUTLINE: This is a multicenter study.
Frozen lymphoblast samples from patients with T-cell acute lymphoblastic leukemia (NOTCH1-mutated and wild type) are assessed for genetic expression profiles and mutations by microarray analysis and activated NOTCH1 protein by western blot analysis.
PROJECTED ACCRUAL: A total of 48 samples from patients will be accrued for this study.
|Study Chair:||Adolfo A. Ferrando, MD, PhD||Herbert Irving Comprehensive Cancer Center|