Molecular Analysis of Retinoblastoma

This study is currently recruiting participants. (see Contacts and Locations)
Verified May 2014 by St. Jude Children's Research Hospital
Sponsor:
Collaborator:
Information provided by (Responsible Party):
St. Jude Children's Research Hospital
ClinicalTrials.gov Identifier:
NCT00718783
First received: July 17, 2008
Last updated: May 1, 2014
Last verified: May 2014
  Purpose

Retinoblastoma is a childhood tumor of the retina that affects approximately 1 in 18,000 children. Retinoblastoma is more prevalent in Central America, where most patients present with advanced intraocular and extraocular disease. Therefore, we have formed collaboration with Central American investigators to provide us with frozen retinoblastoma tumors from patients undergoing routine enucleation. The objective of this protocol is to begin to identify the mutations and gene expression changes that occur in retinoblastoma cells following RB1 gene inactivation in order to more effectively target chemotherapy for treating bilateral retinoblastoma.


Condition Intervention
Retinoblastoma
Other: Tissue samples taken from retinoblastoma
Other: 5 ml peripheral blood

Study Type: Observational
Study Design: Observational Model: Case-Only
Time Perspective: Prospective
Official Title: Molecular Analysis of Retinoblastoma

Resource links provided by NLM:


Further study details as provided by St. Jude Children's Research Hospital:

Primary Outcome Measures:
  • Identify genes exhibiting differential expression within intraocular disease tissue between patients having only intraocular involvement and those having extraocular involvement. [ Time Frame: Indefinite ] [ Designated as safety issue: No ]

Biospecimen Retention:   Samples With DNA
  • Each tumor sample will be immediately transferred to a sterile cryovial and flash frozen in liquid nitrogen.
  • White blood cells will be isolated from the blood sample as a source of genomic DNA.

Estimated Enrollment: 40
Study Start Date: April 2007
Estimated Study Completion Date: June 2015
Estimated Primary Completion Date: June 2015 (Final data collection date for primary outcome measure)
Groups/Cohorts Assigned Interventions
Retinoblastoma Other: Tissue samples taken from retinoblastoma
Tissue banking
Other: 5 ml peripheral blood
White blood cells will be isolated from the blood sample as a source of genomic DNA.

Detailed Description:

This is a biology protocol with no treatment regimen associated. Following routine enucleation of the retinoblastoma affected eye, a portion of the tumor will be removed and flash frozen and sent to St. Jude for RNA and DNA analysis. Some tissue will undergo histopathological analysis and tissue microarrays will be isolated.

  Eligibility

Genders Eligible for Study:   Both
Accepts Healthy Volunteers:   No
Sampling Method:   Non-Probability Sample
Study Population

Patients with retinoblastoma

Criteria

Inclusion Criteria:

  • Must have clinical diagnosis of retinoblastoma (to be confirmed histologically after enucleation)
  • Legal guardians must willing to sign an informed consent indicating that they are aware of this study and its possible benefits. Legal guardians will be given a copy of the consent form.
  • Patient is being seen at this AHOPCA institution:

Exclusion Criteria:

  • Ophthalmologist or oncologist is concerned that sampling will interfere with staging.
  • Diagnosis other than retinoblastoma.
  • Affiliated hospital is unable to provide pathologic evidence of retinoblastoma.
  Contacts and Locations
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below. For general information, see Learn About Clinical Studies.

Please refer to this study by its ClinicalTrials.gov identifier: NCT00718783

Contacts
Contact: Rachel Brennan, MD 1-866-278-5833 info@stjude.org

Locations
Chile
Hospital Calvo Mackenna Recruiting
Santiago, Chile
Contact: Milena Villarroel, MD         
Principal Investigator: Milena Villarroel, MD         
Honduras
Hospital Escuela Bloque Materno Infantil Recruiting
Tegucigalpa, Honduras
Contact: Ligia Fu, MD    222-2322 ext 316    ligiafucarrasco@yahoo.com   
Principal Investigator: Ligia Fu, MD         
Sponsors and Collaborators
St. Jude Children's Research Hospital
Investigators
Principal Investigator: Rachel Brennan, MD St. Jude Children's Research Hospital
  More Information

Additional Information:
No publications provided

Responsible Party: St. Jude Children's Research Hospital
ClinicalTrials.gov Identifier: NCT00718783     History of Changes
Other Study ID Numbers: MARB, 1R01EY018599
Study First Received: July 17, 2008
Last Updated: May 1, 2014
Health Authority: United States: Institutional Review Board

Additional relevant MeSH terms:
Retinoblastoma
Neoplasms, Neuroepithelial
Neuroectodermal Tumors
Neoplasms, Germ Cell and Embryonal
Neoplasms by Histologic Type
Neoplasms
Neoplasms, Glandular and Epithelial
Neoplasms, Nerve Tissue
Retinal Neoplasms
Eye Neoplasms
Neoplasms by Site
Eye Diseases
Retinal Diseases

ClinicalTrials.gov processed this record on August 25, 2014