Effect of the Number of Inseminated Spermatozoa on Subsequent Human Embryonic Development in Vitro
In order to reach fertilization in the context of IVF, the presence of high concentrations of spermatozoa is associated with a higher degree of sperm metabolism and a higher concentration of sperm degradation products, which may adversely affect not only sperm and oocyte viability and the fertilization rate. The effect of a high concentration of sperm used for oocyte insemination appears also to be negative on embryo development (Dumoulin et al 1992*). If that is true, lowering the sperm concentration for oocyte insemination might improve embryo quality and result in a higher implantation rate per embryo. Therefore, we tested the hypothesis that the percentage of 8 cell-embryos on day 3 after IVF is significantly higher (40%) after insemination with a low sperm concentration (150 000/ml spermatozoa) than after insemination with a higher sperm concentration (30%; group 600 000/ml spermatozoa).
|Study Design:||Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single Blind (Subject)
Primary Purpose: Treatment
|Official Title:||Effect of the Number of Inseminated Spermatozoa on Subsequent Human Embryonic Development in Vitro|
- embryo quality [ Time Frame: 36 hours ] [ Designated as safety issue: Yes ]
- implantation rate [ Time Frame: 9 months ] [ Designated as safety issue: Yes ]
|Study Start Date:||August 2006|
Insemination of the oocytes with a lower concentration of spermatozoa.
Insemination with a lower concentration of spermatozoa: 150 000 spermatozoa/ml
No Intervention: 2
Insemination with a normal concentration of spermatozoa.
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