Bioavailability of Golden Rice Carotenoids in Humans
This investigation protocol uses an isotope reference method to evaluate bioavailability of ß-C in rice and its bioconversion to vitamin A.
A well-nourished population will be fed hydroponically grown Golden Rice containing ~ 1 mg of β-carotene at day 8 after a reference dose of vitamin A at day 1. The blood samples will be collected up to 33 days after the doses. The absorption kinetics will be determined by tracking both vitamin A and β-carotene in human serum. Blood responses to Golden Rice β-carotene will be determined and evaluated. The vitamin A value of Golden Rice can thus be quantitatively determined.
|Study Design:||Allocation: Non-Randomized
Endpoint Classification: Efficacy Study
Intervention Model: Single Group Assignment
Masking: Open Label
Primary Purpose: Basic Science
|Official Title:||Bioavailability of Golden Rice Carotenoids in Humans|
- The enrichment of labeled golden rice β-carotene and its conversion to labeled retinol in serum samples after the golden rice meal with 0, 5, or 10 g fat (butter) as compared to the reference dose of vitamin A that is labeled differently. [ Time Frame: 33 days ] [ Designated as safety issue: No ]
|Study Start Date:||August 2004|
|Estimated Study Completion Date:||August 2012|
|Estimated Primary Completion Date:||August 2012 (Final data collection date for primary outcome measure)|
No Intervention: 1
using 10 g fat in the Golden Rice meal
Dietary Supplement: golden rice
An acute dose of cooked golden rice containing ~ 1 mg of ß-carotene
Other Name: golden rice carotenoids
No Intervention: 2
0g dietary fat (butter) eating with the Golden Rice meal
No Intervention: 3
using 5 g fat in the Golden Rice meal
Recently, scientists have genetically engineered "golden rice" that contains 1.6 ug ß-carotene in a gram of dry rice (12). Since the vitamin A equivalency varies from 2 ug (oil dose) to 27 ug (vegetable) carotene to 1 retinol equivalent and the equivalency is matrix dependent, it is necessary to know what the vitamin A equivalency of golden rice beta-carotene is. The determination of the absorption and conversion of golden rice beta-carotene is of importance for designing food programs in many rice-eating regions of the world, where vitamin A deficiency is common.
To evaluate the absorption and intestinal conversion of rice beta-carotene after an acute dose of golden rice, an intrinsically labeled Golden Rice with a labeled reference dose of vitamin A are used. We propose to conduct a pilot study on the US adults with normal vitamin A status (n = 15).
The specific aims of this pilot study are:
- To determine ß-carotene response kinetics following a meal of golden rice with 0, 5, or 10 g fat (butter);
- To determine retinol kinetics from the golden rice and from the labeled vitamin A dose;
- To determine vitamin A value of golden rice ß-carotene in adults with normal vitamin A status. The determination of the absorption and conversion of golden rice ß-carotene is of importance for designing food programs in many rice-eating regions of the world, where vitamin A deficiency is common.
|Contact: guangwen tang, Ph. D.||email@example.com|
|Contact: Lisa Ceglia, M.D.||617-556-3085||lisa.Ceglia@tufts.edu|
|United States, Massachusetts|
|USDA Human Nutrition Research Center on Aging at Tufts Uni||Recruiting|
|Boston, Massachusetts, United States, 02111|
|Contact: Guangwen Tang, Ph.D 617-556-3236 firstname.lastname@example.org|
|Contact: Lisa Ceglia, M.D. 617-556-3085 email@example.com|
|Principal Investigator: Guangwen Tang, Ph. D|
|Principal Investigator:||Guangwen Tang, Ph. D||Tufts University|