Infectious Causes of Uveitis
Uveitis is an inflammatory disease of one or both eyes. Uveitis will in severe cases lead to permanent loss of vision/blindness on the affected eye. Uveitis can be caused by autoimmune disease, infections and rarely trauma, but 50 % of the cases remain of unknown origin. Intraocular infections are from previous studies, known to be responsible for approximately 40 % of the cases of severe forms of uveitis. Most infectious causes of uveitis have the potential to be treated with antibiotic.
Most infectious causes of uveitis are under normal condition only possible to detect by using very specific detection methods. Further more it is often necessary to study a sample from with-in the eye (vitrectomy to get a proper diagnosis. The knowledge about witch kind of infections that causes uveitis, are therefore limited to the kind of infections that are tested for.
Our hypothesis are that infections are a frequent cause of severe uveitis in Denmark, and that by using a broad diagnostic approach it is possible to identify new or less recognized infections agents that are associated with uveitis. By this study we want to describe the prevalence and distribution of infections among patients with severe uveitis. In this study are we only including patients with a severe form of uveitis from a referral-hospital setting, where vitrectomy is done for a diagnostic purpose.
The patients will undergo a standard diagnostic examination including the most common infectious causes of uveitis. Beside this we will look for a broad range of infection, which has previously been described in the litera-ture to cause uveitis, but are not normally tested for. Further more, we will use broad ranged molecular diagnostic methods to look for new previously unknown bacterial causes of uveitis. Such study has not previously been done previously in Denmark. As control group are included patients that undergo vitrectomy for due either one of the two diseases "epiretinal fibrosis or "macula hole". All patients and controls will be interview using a standardized questionnaire about risk factors for acquiring these infections.
|Study Design:||Observational Model: Cohort
Time Perspective: Prospective
|Official Title:||Infectious Causes of Uveitis, With Special Focus on Infection With Leptospira|
- Prevalence and distribution of intraocular infections in the study group [ Time Frame: September 2010 ] [ Designated as safety issue: No ]
- The value of non-specific molecular diagnostic methods for detection of bacteria in the study group [ Time Frame: September 2010 ] [ Designated as safety issue: No ]
Biospecimen Retention: Samples With DNA
Corpus vitreum (vitreus body) Aqua from the anterior champer Whole blood in dry container Whole blood in container containing EDTA Whole blood in QuantiFERON test KIT for tuberculosis Urine
|Study Start Date:||April 2008|
|Study Completion Date:||September 2010|
|Primary Completion Date:||September 2010 (Final data collection date for primary outcome measure)|
Patients suffering from uveitis, who have vitrectomy performed for diagnostic purpose
Patients suffering from either "Epiretinal fibrosis" og "Macula hole" who have vitrectomy performed for curative reasons
All patients included eye examination that includes split-lamp examination with semi-quantification evaluation of the inflammation, fundoscopy incl. picture, and angiography.
Hemoglobin (Hb), Erythrocyte-volume fraction (EVF), Middle cell volume (MCV), middelcell hemoglobin concentration (MCHC), Trombocyte
- Alanin-aminotransferase (ALAT), S-albumin, S-bilirubin (total), Protrombin complex, INR.
- Na, K, creatinin,
- CRP, Leukocyte and differential count
- ACE (Angiotensin Converting Enzyme)
X-ray of thorax
The biopsies taken from the patient are in the routine program are patients tested for intraocular infections with Herpes Simplex Virus, Cytomegalovirus, Varicella Zoster virus, Epstein-Barr virus and toxoplasma with PCR and serology on serum. Test for others infections like Candida spp. and Toxocare are done if clini-cally indicated. Other test includes cytopathological test of the corpus vitreum for cancer cells and serological test for Syphilis.
Beside the above mentioned test will we in this study test for:
Infections with Leptospira, Bartonella, Brucella, Mycobacterium tuberculosis, Leptospira, Toxocare, Borrelia, HIV.
We will use unspecific molecular test for Bacteria under a special setup to avoid DNA contamination. Culture of Leptospira will be done on urine samples.
Please refer to this study by its ClinicalTrials.gov identifier: NCT00676624
|Glostrup, Denmark, 2600|
|Principal Investigator:||Steen Villumsen, MD PhD stud||Statens Serum Institut|
|Study Chair:||Morten la Cour, MD DMSc||Glostrup Hospital|
|Study Chair:||Karen A Krogfelt, Prof. PhD||Statens Serum Institut|
|Study Chair:||Helle J Fuchs, MD||Glostrup Hospital|
|Study Chair:||Jan U Prause, MD Prof DMSC||University of Copenhagen|
|Study Chair:||Henrik V Nielsen, PhD||Statens Serum Institut|
|Study Chair:||Lars P Nielsen, MD||Statens Serum Institut|
|Study Chair:||Vibeke Thomsen, Cand Scient||Statens Serum Institut|
|Study Chair:||Anne-Mette Lebech, MD, DMSc||Hvidovre University Hospital|
|Study Chair:||Jorgen S Jensen, MD DMSc||Statens Serum Institut|