• Number of Patients With In Vivo Expansion of Infused Allogeneic Natural Killer (NK) Cell Product [ Time Frame: Day 12-14 ] [ Designated as safety issue: No ]
  Detection of an absolute donor derived cell count of > or = 100 cells/mL after NK cell infusion.
  
  

• Number of Patients Per Disease Response [ Time Frame: 1 Month After Natural Killer Cell Infusion (Day 30) ] [ Designated as safety issue: No ]
  Response Evaluation Criteria in Solid Tumors (RECIST) criteria: Complete Response (CR)-Disappearance of all target lesions (TL); Partial Response (PR)-< or = 30% decrease in the sum of the longest diameter (LD) of TL, reference baseline sum LD; Stable Disease (SD)-Neither sufficient shrinkage to qualify for PR nor sufficient increase to qualify for PD, reference the smallest sum LD since the treatment started; Progressive Disease (PD)- < or = 20% increase in the sum of the LD of TL, reference the smallest sum LD recorded since treatment started or appearance of < or = 1 new lesion.
  
  
• Median Number of Days to Progression [ Time Frame: From date of first treatment to disease progression ] [ Designated as safety issue: No ]
  Median number of days from first date of treatment to date of disease progression (appearance of new metastatic lesions or objective tumor progression). Defined by computated tomography (CT) imaging based on Response Evaluation Criteria In Solid Tumors (RECIST): Progressive Disease (PD) > or = 20% increase in sum of all target or any new lesions.
  
  
• Median Overall Survival Number of Days Patients Alive After Treatment [ Time Frame: From first date on-study (treatment) to date of death ] [ Designated as safety issue: No ]
  Median number of days patients alive from date of treatment to date of death or date of last follow-up if censored.
  
  

OBJECTIVES:

Primary

• To evaluate the in vivo expansion of an infused allogeneic natural killer (NK) cell product following a preparative regimen comprising cyclophosphamide, fludarabine phosphate, and total-body irradiation in treating patients with recurrent and/or metastatic ovarian, fallopian tube, or primary peritoneal cancer.

Secondary

• To characterize the quantitative and qualitative toxicities of this treatment regimen.
• To estimate disease response (complete or partial response) or clinical benefit (stable disease for > 6 months) as measured by Response Evaluation Criteria in Solid Tumours (RECIST) criteria.
• To estimate time to progression and overall survival.
• To estimate the association between clinical response and donor/recipient KIR ligand matching status.

Tertiary

• To evaluate immune activation of the in vivo expanded haploidentical allogeneic NK cells and its effect on the immune system.

OUTLINE:

• Preparative regimen: Patients receive fludarabine phosphate IV on days 6 to 2 preceding natural killer (NK) cell infusion and cyclophosphamide IV on days 5 and 4 preceding NK cell infusion. Patients also undergo total-body irradiation on day 1 preceding NK cell infusion.
• Allogeneic natural killer (NK) cell administration and aldesleukin: Patients receive aldesleukin-activated haploidentical allogeneic NK cells intravenously (IV) on day 0. Beginning 4-6 hours after allogeneic NK cell infusion, patients receive aldesleukin subcutaneously (SC) 3 times a week for 6 doses.

Patients achieving any initial response (complete or partial response) or a clinical benefit (stable disease for > 6 months) who progress after 6 months may receive 1 re-treatment course as above.

Blood samples are collected at baseline, on days 0, 7, 14, and 28, and then at 2 and 3 months post NK cell infusion for cytokine measurements, immunophenotyping, functional analyses, and testing for persistence of donor cells.

After completion of study treatment, patients are followed periodically for at least 1 year.