Comparison of the Effects on Promoting Corneal Epithelial Wound Healing Between Human Auto-Serum and Cord Blood Serum

This study has been completed.
Sponsor:
Information provided by:
National Taiwan University Hospital
ClinicalTrials.gov Identifier:
NCT00598299
First received: January 10, 2008
Last updated: November 2, 2008
Last verified: October 2008
  Purpose

Human serum eye drops have been successfully used in the treatment of severe ocular surface disorders and the enhancement of corneal wound healing. Umbilical cord serum is also proven to be effective in treatment of dry eye and persistent corneal epithelial defects. However, there are limited studies comparing the corneal epithelial wound healing promoting effects between these two blood derived products. The purpose of this study is to test the corneal epithelial wound healing promoting effects between auto serum and human cord blood serum. Primary cultured bovine corneal epithelial cells were used as the model to investigate wound healing, cell proliferation and migration by means of scratch corneal wound healing assay evaluation, MTS assay and Boyden chamber migration assay in response to human serum and umbilical cord serum. The concentrations of EGF, TGF-β1, and fibronectin were also compared between human serum and umbilical cord serum with ELISA kits.


Condition Intervention
Eye Injuries
Procedure: venous puncture
Other: obtain serum from perripheral blood or cord blood

Study Type: Observational
Study Design: Observational Model: Case Control
Time Perspective: Prospective
Official Title: Department of Ophthalmology, National Taiwan University Hospital

Resource links provided by NLM:


Further study details as provided by National Taiwan University Hospital:

Estimated Enrollment: 100
Study Start Date: April 2007
Study Completion Date: April 2008
Groups/Cohorts Assigned Interventions
A
Healthy adult serum
Procedure: venous puncture
venous puncture
Other: obtain serum from perripheral blood or cord blood
obtain serum from perripheral blood or cord blood
B
cord blood serum
Procedure: venous puncture
venous puncture
Other: obtain serum from perripheral blood or cord blood
obtain serum from perripheral blood or cord blood

Detailed Description:

Human serum eye drops have been successfully used clinically in the treatment of severe ocular surface disorders and the enhancement of corneal wound healing. Umbilical cord serum is also proven to be effective in treatment of dry eye and persistent corneal epithelial defects. However, there are limited studies comparing the corneal epithelial wound healing promoting effects between these two blood derived products. The purpose of this study is to test the corneal epithelial wound healing promoting effects between auto serum and human cord blood serum. Primary cultured bovine corneal epithelial cells were used as the model to investigate wound healing, cell proliferation and migration by means of scratch corneal wound healing assay evaluation, MTS assay and Boyden chamber migration assay in response to human serum and umbilical cord serum. The concentrations of EGF, TGF-β1, and fibronectin were also compared between human serum and umbilical cord serum with ELISA kits.

  Eligibility

Ages Eligible for Study:   18 Years to 60 Years
Genders Eligible for Study:   Both
Accepts Healthy Volunteers:   Yes
Sampling Method:   Non-Probability Sample
Study Population

heahty adult volunteers and donated cord blood

Criteria

Inclusion Criteria:

healthy volunteers

Exclusion Criteria:

unhealthy volunteers

  Contacts and Locations
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Please refer to this study by its ClinicalTrials.gov identifier: NCT00598299

Locations
Taiwan
National Taiwan University Hospital
Taipei, Taiwan
Sponsors and Collaborators
National Taiwan University Hospital
Investigators
Study Chair: Fung-Rong Hu, MD National Taiwan University Hospital
  More Information

No publications provided

Responsible Party: Wei-Li Chen, Department of Ophthalmology, National Taiwan University Hospital
ClinicalTrials.gov Identifier: NCT00598299     History of Changes
Other Study ID Numbers: 20072037R
Study First Received: January 10, 2008
Last Updated: November 2, 2008
Health Authority: Taiwan: Department of Health

Keywords provided by National Taiwan University Hospital:
corneal epithelium

Additional relevant MeSH terms:
Eye Injuries
Wounds and Injuries
Eye Diseases
Facial Injuries
Craniocerebral Trauma

ClinicalTrials.gov processed this record on July 20, 2014