Comparison of the Effects on Promoting Corneal Epithelial Wound Healing Between Human Auto-Serum and Cord Blood Serum
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Purpose
Human serum eye drops have been successfully used in the treatment of severe ocular surface disorders and the enhancement of corneal wound healing. Umbilical cord serum is also proven to be effective in treatment of dry eye and persistent corneal epithelial defects. However, there are limited studies comparing the corneal epithelial wound healing promoting effects between these two blood derived products. The purpose of this study is to test the corneal epithelial wound healing promoting effects between auto serum and human cord blood serum. Primary cultured bovine corneal epithelial cells were used as the model to investigate wound healing, cell proliferation and migration by means of scratch corneal wound healing assay evaluation, MTS assay and Boyden chamber migration assay in response to human serum and umbilical cord serum. The concentrations of EGF, TGF-β1, and fibronectin were also compared between human serum and umbilical cord serum with ELISA kits.
| Condition | Intervention |
|---|---|
|
Eye Injuries |
Procedure: venous puncture Other: obtain serum from perripheral blood or cord blood |
| Study Type: | Observational |
| Study Design: | Observational Model: Case Control Time Perspective: Prospective |
| Official Title: | Department of Ophthalmology, National Taiwan University Hospital |
| Estimated Enrollment: | 100 |
| Study Start Date: | April 2007 |
| Study Completion Date: | April 2008 |
| Groups/Cohorts | Assigned Interventions |
|---|---|
|
A
Healthy adult serum
|
Procedure: venous puncture
venous puncture
Other: obtain serum from perripheral blood or cord blood
obtain serum from perripheral blood or cord blood
|
|
B
cord blood serum
|
Procedure: venous puncture
venous puncture
Other: obtain serum from perripheral blood or cord blood
obtain serum from perripheral blood or cord blood
|
Detailed Description:
Human serum eye drops have been successfully used clinically in the treatment of severe ocular surface disorders and the enhancement of corneal wound healing. Umbilical cord serum is also proven to be effective in treatment of dry eye and persistent corneal epithelial defects. However, there are limited studies comparing the corneal epithelial wound healing promoting effects between these two blood derived products. The purpose of this study is to test the corneal epithelial wound healing promoting effects between auto serum and human cord blood serum. Primary cultured bovine corneal epithelial cells were used as the model to investigate wound healing, cell proliferation and migration by means of scratch corneal wound healing assay evaluation, MTS assay and Boyden chamber migration assay in response to human serum and umbilical cord serum. The concentrations of EGF, TGF-β1, and fibronectin were also compared between human serum and umbilical cord serum with ELISA kits.
Eligibility| Ages Eligible for Study: | 18 Years to 60 Years |
| Genders Eligible for Study: | Both |
| Accepts Healthy Volunteers: | Yes |
| Sampling Method: | Non-Probability Sample |
heahty adult volunteers and donated cord blood
Inclusion Criteria:
healthy volunteers
Exclusion Criteria:
unhealthy volunteers
Contacts and Locations More Information
No publications provided
| Responsible Party: | Wei-Li Chen, Department of Ophthalmology, National Taiwan University Hospital |
| ClinicalTrials.gov Identifier: | NCT00598299 History of Changes |
| Other Study ID Numbers: | 20072037R |
| Study First Received: | January 10, 2008 |
| Last Updated: | November 2, 2008 |
| Health Authority: | Taiwan: Department of Health |
Keywords provided by National Taiwan University Hospital:
|
corneal epithelium |
Additional relevant MeSH terms:
|
Eye Injuries Eye Diseases Facial Injuries Craniocerebral Trauma Wounds and Injuries |
ClinicalTrials.gov processed this record on May 23, 2013