Chromosomal and Morphological Structure of Mature and Immature Male Germ Cells and Recognition of IL-18 in Testicular Tissue of Azoospermic Men

This study has been completed.
Sponsor:
Information provided by:
Assaf-Harofeh Medical Center
ClinicalTrials.gov Identifier:
NCT00544089
First received: October 14, 2007
Last updated: October 15, 2007
Last verified: March 2006
  Purpose

To develop a biotechnological assay which will be able to recognize germ cells of the spermatogenic lineand and to identify the chromosomal and morphological structure of mature and immature male germ cells, in cases of of sperm production disorders(azoospermic patients).

To recognize IL-18 in testicular tissue of azoospermic men compared to control group as it has been suggested that this cytokine may serve as a paracrine defense factor in the male gonad associated to spermatogenesis.


Condition
Infertility

Study Type: Observational
Study Design: Time Perspective: Prospective

Resource links provided by NLM:


Further study details as provided by Assaf-Harofeh Medical Center:

Enrollment: 25907
Study Start Date: March 2006
Study Completion Date: September 2007
Detailed Description:

A new biotechnological assay was developed by adapting an existing computerized system which scans cell morphology and chromosomal ploidy in the same cell of hematologic line using digital technology, to recognize germ cells of the testis by their morphology and cytogenetics. Testicular tissues which obtained from 15 men were enzymatically digested and the cells received were fixed on slides. The cells were stained by Giemsa May Grinwald staining and scanned automatically in a bright field for cell morphology. Fluorescence in situ hybridization (FISH) using centromeric probes for chromosomes X, Y and 18 was performed on the same slide after destaining. The cells were automatically scanned and classified according to the ploidy of the chromosome signals. The presence of IL-18 in the same men's testis was examined parallelly, by immunohistochimical staining using specific antibody for human IL-18.

  Eligibility

Ages Eligible for Study:   22 Years to 39 Years
Genders Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • Non-obstructive azoospermic patients

Exclusion Criteria:

  • Obstructive azoospermia
  Contacts and Locations
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Please refer to this study by its ClinicalTrials.gov identifier: NCT00544089

Locations
Israel
Infertility unit, Asaf-Harofeh Medical Center
Zrifin, Israel
Sponsors and Collaborators
Assaf-Harofeh Medical Center
Investigators
Principal Investigator: Raphael Ron-el, professor Assaf-Harofeh Medical Canter
  More Information

No publications provided

ClinicalTrials.gov Identifier: NCT00544089     History of Changes
Other Study ID Numbers: 37/06
Study First Received: October 14, 2007
Last Updated: October 15, 2007
Health Authority: Israel: Ethics Commission

Keywords provided by Assaf-Harofeh Medical Center:
azoospermia FISH IL-18 TESTICULAR BIOPSY

Additional relevant MeSH terms:
Infertility
Genital Diseases, Male
Genital Diseases, Female

ClinicalTrials.gov processed this record on August 19, 2014