Chromosomal and Morphological Structure of Mature and Immature Male Germ Cells and Recognition of IL-18 in Testicular Tissue of Azoospermic Men
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Purpose
To develop a biotechnological assay which will be able to recognize germ cells of the spermatogenic lineand and to identify the chromosomal and morphological structure of mature and immature male germ cells, in cases of of sperm production disorders(azoospermic patients).
To recognize IL-18 in testicular tissue of azoospermic men compared to control group as it has been suggested that this cytokine may serve as a paracrine defense factor in the male gonad associated to spermatogenesis.
| Condition |
|---|
|
Infertility |
| Study Type: | Observational |
| Study Design: | Time Perspective: Prospective |
| Enrollment: | 25907 |
| Study Start Date: | March 2006 |
| Study Completion Date: | September 2007 |
A new biotechnological assay was developed by adapting an existing computerized system which scans cell morphology and chromosomal ploidy in the same cell of hematologic line using digital technology, to recognize germ cells of the testis by their morphology and cytogenetics. Testicular tissues which obtained from 15 men were enzymatically digested and the cells received were fixed on slides. The cells were stained by Giemsa May Grinwald staining and scanned automatically in a bright field for cell morphology. Fluorescence in situ hybridization (FISH) using centromeric probes for chromosomes X, Y and 18 was performed on the same slide after destaining. The cells were automatically scanned and classified according to the ploidy of the chromosome signals. The presence of IL-18 in the same men's testis was examined parallelly, by immunohistochimical staining using specific antibody for human IL-18.
Eligibility| Ages Eligible for Study: | 22 Years to 39 Years |
| Genders Eligible for Study: | Male |
| Accepts Healthy Volunteers: | Yes |
Inclusion Criteria:
- Non-obstructive azoospermic patients
Exclusion Criteria:
- Obstructive azoospermia
Contacts and Locations| Israel | |
| Infertility unit, Asaf-Harofeh Medical Center | |
| Zrifin, Israel | |
| Principal Investigator: | Raphael Ron-el, professor | Assaf-Harofeh Medical Canter |
More Information
No publications provided
| ClinicalTrials.gov Identifier: | NCT00544089 History of Changes |
| Other Study ID Numbers: | 37/06 |
| Study First Received: | October 14, 2007 |
| Last Updated: | October 15, 2007 |
| Health Authority: | Israel: Ethics Commission |
Keywords provided by Assaf-Harofeh Medical Center:
|
azoospermia FISH IL-18 TESTICULAR BIOPSY |
Additional relevant MeSH terms:
|
Infertility Genital Diseases, Male Genital Diseases, Female |
ClinicalTrials.gov processed this record on June 18, 2013