Plasma B-Type Natriuretic Peptide Concentrations in Preterm Infants < 28 Weeks
The aim of the study was to evaluate BNP in preterm infants < 28 weeks on the second day of life, when it is still unknown, if a patent ductus arteriosus is hemodynamic significant or not. We hypothesized that high plasma BNP concentrations on day 2 are associated with the need of PDA intervention in the further course.
Ductus Arteriosus, Patent
Natriuretic Peptide Brain
|Study Design:||Observational Model: Defined Population
Primary Purpose: Screening
Time Perspective: Longitudinal
|Study Start Date:||August 2005|
|Study Completion Date:||April 2007|
From the study infants blood samples (0.1ml) were collected in K-EDTA microtubes within 24 to 48 hours of age through umbilical artery catheter aspiration or routine venous puncture. The samples were spun at 7000 rpm for 3 minutes. Platelet-free plasma was stored at -20°C until BNP analysis of all collected samples was carried out. The BNP assay was performed using a fully automated immunoassay system ADVIA method for the Centaur System (Bayer Diagnostic Division).
In preterm infants who were treated with indomethacin or surgical ligation we collected additional blood samples 2-4 hours before intervention and 24 hours after the completion of the ductus treatment.
|Department of Neonatology, Charite Virchow Hospital, Medical University Berlin|
|Berlin, Germany, 13353|
|Study Director:||Christoph Czernik, MD||Department of Neonatology, Charite Virchow Hospital, Medical University Berlin|