Use of Biopsy Punch for Skin Biopsies for Fibroblasts Culturing

This study has been completed.
Sponsor:
Information provided by:
National Institutes of Health Clinical Center (CC)
ClinicalTrials.gov Identifier:
NCT00340912
First received: June 19, 2006
Last updated: October 2, 2009
Last verified: October 2009
  Purpose

Fibroblasts can be cultured from human skin biopsies using fetal bovine serum and artificial growth medium. Fibroblasts cultured in this way retain the genetic characteristics of their donor but can be used for experiments where study conditions are much more precisely controlled than they can be in in vivo studies. We plan to culture fibroblasts from Pima Indians for two purposes. Firstly, to look for an in vitro system to measure insulin resistance, the underlying abnormality in Pima diabetics. Secondly, to look for biochemical markers of diabetes or obesity in Pimas employing two dimensional electrophoresis techniques.


Condition
Fibroblasts

Study Type: Observational
Official Title: Use of Biopsy Punch for Skin Biopsies for Fibroblasts Culturing

Further study details as provided by National Institutes of Health Clinical Center (CC):

Estimated Enrollment: 500
Study Start Date: December 1988
Primary Completion Date: December 1988 (Final data collection date for primary outcome measure)
Detailed Description:

Fibroblasts can be cultured from human skin biopsies using fetal bovine serum and artificial growth medium. Fibroblasts cultured in this way retain the genetic characteristics of their donor but can be used for experiments where study conditions are much more precisely controlled than they can be in in vivo studies. We plan to culture fibroblasts from Pima Indians for two purposes: 1) to look for an in vitro system to measure insulin resistance, the underlying abnormality in Pima diabetics; and 2) to look for biochemical markers of diabetes or obesity in Pimas employing two-dimensional electrophoresis techniques.

  Eligibility

Ages Eligible for Study:   18 Years to 50 Years
Genders Eligible for Study:   Both
Accepts Healthy Volunteers:   No
Criteria
  • INCLUSION CRITERIA:

We will include Pima Indians who do not meet any of the exclusion criteria.

EXCLUSION CRITERIA:

We will exclude any seriously ill individuals, any known to have a bleeding diathesis or to be immunosuppressed or subjects who are carriers of HIV or HBV.

Children will be excluded, as the age range for this study's participants is 18-50.

Pregnant women will be excluded on the basis of a urinary pregnancy test.

All other subjects are potentially included.

  Contacts and Locations
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Please refer to this study by its ClinicalTrials.gov identifier: NCT00340912

Locations
United States, Arizona
NIDDK, Phoenix
Phoenix, Arizona, United States, 85014
Sponsors and Collaborators
  More Information

No publications provided

ClinicalTrials.gov Identifier: NCT00340912     History of Changes
Other Study ID Numbers: 9999880215, OH88-DK-0215
Study First Received: June 19, 2006
Last Updated: October 2, 2009
Health Authority: United States: Federal Government

Keywords provided by National Institutes of Health Clinical Center (CC):
Skin Biopsy

ClinicalTrials.gov processed this record on October 01, 2014