Anti-Pseudomonas Immune Status and Dendritic Cell Preparation in Normal Individuals
This study has been withdrawn prior to enrollment.
(research priority change)
Information provided by:
Weill Medical College of Cornell University
First received: April 27, 2006
Last updated: September 24, 2010
Last verified: September 2010
This protocol aims to collect human peripheral blood mononuclear cells for the production of dendritic cells (DC). There are two ways to collect human peripheral blood mononuclear cells in this protocol. One is by using the method of leukapheresis from normal healthy adults. The other is by drawing the blood from normal healthy adults and then getting the buffy coats from the blood.
||Observational Model: Case Control
Time Perspective: Prospective
||Anti-Pseudomonas Immune Status and Dendritic Cell Preparation in Normal Individuals
| Estimated Enrollment:
| Study Start Date:
| Estimated Study Completion Date:
One objective of these studies on DC is to complete a preclinical development for a human study of a candidate anti-Pseudomonas aeruginosa vaccine consisting of autologous dendritic cells primed with heat-inactivated P. aeruginosa (PA) strain POA1 and expressing the T-cell costimulatory molecule CD40 ligand (CD40L). The other objective is to complete a preclinical development for a human study of a candidate anti-anthrax vaccine. Prior to testing the vaccine in humans, the Food and Drug Administration will require preclinical and Good Manufacturing Practice (GMP) practice batches of dendritic cells. The intent of the leukapheresis or collecting the buffy coats is to provide the biological materials for this purpose.
|Ages Eligible for Study:
||18 Years and older
|Genders Eligible for Study:
|Accepts Healthy Volunteers:
Group A is for leukapheresis and Group B is for buffy coats collection. We will use either Group A or Group B or both to collect mononuclear cells. The source of potential subjects in both groups will be normal individuals recruited from the population of healthy individuals in the New York metropolitan area and elsewhere. Accrual will be random with no bias as to gender or racial/ethnic group. Nevertheless, because the accrual process will be random, it is possible that among the study individuals there may be differences in the proportion of males and females, and racial/ethnic groups. The study volunteers will be recruited through IRB approved advertisements placed in local newspapers and on fliers to be circulated throughout the New York Metropolitan area and elsewhere. All efforts will be made to recruit women and minorities.
- All study subjects must be capable of providing informed consent.
- Normal male or female, age 18 and older.
- Not taking corticosteroids
- Not taking any prescription or over-the-counter medication, except acetaminophen (tylenol)
- Participants of Group A must have adequate peripheral veins for leukapheresis
- Study subjects should not be taking experimental medications.
- Females cannot be pregnant.
- Individuals who do not meet the inclusion criteria will be unable to participate in the protocol.
- Individuals with active infection in the 3 weeks prior to beginning the protocol will not be able to participate.
- Individuals with evidence of significant cardiac, pulmonary, renal, endocrine, central nervous system, major psychiatric disorder that would prevent compliance or substance abuse, musculoskeletal disease or immunodeficiency disease, (including evidence of human immunodeficiency virus infection, HIV) will be unable to participate in the protocol.
- Individuals participating in any other experimental clinical studies.
- Women who are pregnant or nursing.
- Active or recent drug users
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Please refer to this study by its ClinicalTrials.gov identifier: NCT00320164
Weill Medical College of Cornell University
||Neil R. Hackett, Ph.D.
||Weill Medical College of Cornell University
No publications provided
||Department of Genetic Medicine, Weill Cornell Medical College
History of Changes
|Other Study ID Numbers:
|Study First Received:
||April 27, 2006
||September 24, 2010
||United States: Institutional Review Board
Keywords provided by Weill Medical College of Cornell University:
ClinicalTrials.gov processed this record on November 27, 2014