Inclusion Criteria:

1. Participants should be of age eighteen years or older
2. Participant has painful bladder syndrome and a score of 8 or greater on the painful bladder syndrome symptom index (O'Leary-Sant)
3. Participant should have frequency and urgency of micturition and bladder pain for at least 6 months
4. Participant should have voids at least 8 times during a 24 hour period and at least once during the night as documented in the voiding diary and questionnaire.
5. Urine culture should not show any evidence of urinary tract infection.
6. Participant should be able to understand, speak, and read English.
7. Participant should be willing to take part in the study, including signing this form after carefully reading it.
8. Participant consents to use a medically acceptable method of birth control throughout the entire study period and for four weeks after the study is completed. Medically acceptable methods of contraception that may be used by the study participants and/or their partners include abstinence, birth control pills or patches, diaphragm with spermicide, intrauterine device (IUD), condom and vaginal spermicide, surgical sterilization, vasectomy, or progestin implants or injections.
9. Failure of other treatments for PBS/IC like pentosan polysulfate (failure is defined as a score of +1 [slightly improved] or less on a global assessment response [GRA] 23 question to previous therapy)

Exclusion Criteria:

1. Participants who have undergone cystoscopy within 4 weeks of screening visit
2. Participants had other treatment given into the bladder in the past 4 weeks
3. Participants have used a new drug in the past 4 weeks which could affect bladder symptoms (some antidepressants, anticholinergics, antihistamines)
4. Past history of treatment with cyclophosphamide
5. A positive pregnancy test at the time of screening
6. Currently breast feeding
7. History of uterine, cervical, or vaginal cancer during the past 3 years
8. History of significant vaginitis
9. History of major surgery in the last 6 months
10. Positive tests for HIV, hepatitis B virus (HBV), and hepatitis C virus (HCV)
11. History of immune deficiency diseases
12. History of bleeding disorders
13. History of serious social, mental, or medical conditions that would stop patient from taking part in the study
14. History of alcohol or drug abuse within the last 5 years
15. Participants who have history of prostate cancer or are being treated for chronic bacterial prostatitis
16. History of liver disease or significant medical problem which the investigator considers a risk for patient to be a part of the study
17. History of any of the following: neurogenic bladder radiation to pelvic area, inflammation of the bladder wall because of tuberculosis, schistosomiasis, bladder or ureteric calculi, or active genital herpes within 3 months of screening
18. Known hypersensitivity to one of the agents used in the intravesical instillation
19. Use of any investigational drug or device in the last 6 months
20. Participants who are unwilling or unable to abide by the requirements of the study