Effect of Solar-Simulated Ultraviolet Radiation on Gene Expression in Unprotected and Sunscreen-Protected Skin of Healthy Adults With Fitzpatrick Skin Type II
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Purpose
RATIONALE: Testing of skin that has been exposed to artificial sunlight may help in understanding the genetic processes involved in the development of skin cancer.
PURPOSE: This trial is studying the effect of solar-simulated ultraviolet radiation on skin with or without sunscreen in healthy adults with skin that burns easily after only slight tanning during sun exposure.
| Condition | Intervention |
|---|---|
|
Non-melanomatous Skin Cancer Precancerous Condition |
Genetic: microarray analysis Procedure: biopsy Procedure: evaluation of cancer risk factors |
| Study Type: | Observational |
| Official Title: | Transcriptional Profiling of the Epidermal Response to Solar-Simulated Ultraviolet Radiation |
| Study Start Date: | February 2004 |
OBJECTIVES:
- Determine the global gene expression profiles in epidermal tissue of healthy fair-skinned adults with Fitzpatrick skin type II after exposure to known doses of solar-simulated ultraviolet radiation (ssUVR) (UVA).
- Determine the ability of an FDA-standardized sunscreen to alter ssUVR-induced transcription profiles in vivo in these participants.
- Determine whether the transcriptional profiles of keratinocytes exposed to UVR in cell culture are comparable to the profiles of keratinocytes in skin after similar levels of UVR exposure.
OUTLINE: This is a pilot, dose-response study followed by an in vivo transcriptional profiling study in 2 different groups.
- Pilot study (verification of ultraviolet radiation dose response): Participants are initially exposed to solar-simulated ultraviolet radiation (ssUVR) (UVA) on the back to determine the minimum erythema dose (MED). Between 22-24 hours after exposure, participants undergo shave biopsy from each of the 9 UV exposure sites and 1 unexposed skin site.
- Group 1 (determination of ssUVR and UVA in vivo transcriptional profiles): On day 1, participants are initially exposed to ssUVR on the back to determine the MED. On day 2, participants are exposed to ssUVR (3 sites) and UVA (3 sites) at the MED on the buttocks. Between 22-24 hours after exposure (day 3), participants undergo shave biopsy from each of the 6 UV exposure sites and 2 unexposed skin sites.
- Group 2 (determination of sunscreen-protected in vivo transcriptional profiles): On day 1, participants are initially exposed to ssUVR on the back to determine the MED. On day 2, participants are exposed to ssUVR on unprotected skin (3 sites) and sunscreen-protected skin (3 sites) at the MED on the buttocks. Between 22-24 hours after exposure (day 3), participants undergo shave biopsy from each of the 6 UV exposure sites and 2 unexposed skin sites.
Biopsies from all participants are analyzed by microarray analysis. One of the unexposed epidermal samples is used for primary keratinocyte culture.
PROJECTED ACCRUAL: A total of 6-56 participants (6 for the pilot study and 50 [25 per group] for transcriptional profiling) will be accrued for this study within 2 months.
Eligibility| Ages Eligible for Study: | 18 Years to 45 Years |
| Genders Eligible for Study: | Both |
| Accepts Healthy Volunteers: | Yes |
DISEASE CHARACTERISTICS:
- Healthy, fair-skinned adults
Fitzpatrick skin type II by history and physical exam
- Skin easily burns, and tans only slightly, after sun exposure
Test site for solar-simulated ultraviolet radiation exposure (buttocks) devoid of sunburn, suntan, scars, active dermal lesions, and uneven skin tones
- Nevi allowed at physician discretion
- Excess hair must be clipped or shaved
- No prior nonmelanoma skin cancer, melanoma, or dysplastic nevi
PATIENT CHARACTERISTICS:
Age
- 18 to 45
Performance status
- Not specified
Life expectancy
- Not specified
Hematopoietic
- Not specified
Hepatic
- Not specified
Renal
- Not specified
Other
- No prior malignancy
- No prior phototoxic, photoallergic, or other abnormal responses to sunlight
- No prior allergic reaction to sunscreen or lidocaine
- No underlying disease that is known to cause immunosuppression (e.g., HIV, cancer, or post-organ transplantation)
- No situation that would preclude study compliance
- Not pregnant or nursing
PRIOR CONCURRENT THERAPY:
Biologic therapy
- Not specified
Chemotherapy
- Not specified
Endocrine therapy
- Not specified
Radiotherapy
- Not specified
Surgery
- Not specified
Other
- More than 6 months since prior topical or systemic medication producing abnormal sunlight responses
- No concurrent medications with > 1% incidence of sun-related toxic effects
- No concurrent medications associated with abnormal light response
- No concurrent immunosuppressants
- No other concurrent ultraviolet radiation (e.g., sunlight or tanning bed) to the epidermal test site
Contacts and Locations| United States, Maryland | |
| Warren Grant Magnuson Clinical Center - NCI Clinical Studies Support | |
| Bethesda, Maryland, United States, 20892-1182 | |
| Study Chair: | Jonathan C. Vogel, MD | NCI - Dermatology Branch |
More Information
Additional Information:
No publications provided
| ClinicalTrials.gov Identifier: | NCT00099112 History of Changes |
| Obsolete Identifiers: | NCT00077740 |
| Other Study ID Numbers: | CDR0000357437, NCI-04-C-0120 |
| Study First Received: | December 8, 2004 |
| Last Updated: | February 1, 2010 |
| Health Authority: | United States: Federal Government |
Keywords provided by National Cancer Institute (NCI):
|
basal cell carcinoma of the skin squamous cell carcinoma of the skin actinic keratosis |
Additional relevant MeSH terms:
|
Skin Neoplasms Precancerous Conditions Neoplasms by Site Neoplasms Skin Diseases |
ClinicalTrials.gov processed this record on June 17, 2013